Allelic frequency of PCR-RFLP type of the BoLA-DRB3 in Japanese Holstein herds and the relation to mastitis

• Published in: Animal science journal 2008-08, Vol.79 (4), p.409-416
• Main Authors: Yoshida, T.(Nippon Veterinary and Life Science Univ., Musashino, Tokyo (Japan)), Mukoyama, H, Furuta, H, Holmes, C.W, Kosugiyama, M, Tomogane, H
• Format: Article
• Language: English
• Subjects: BoLA-DRB3; Cattle; Cellular biology; CONTEO DE CELULAS SOMATICAS; DAIRY COWS; GENE; GENES; Genetics; MAMMITE; MASTITIS; METHODE STATISTIQUE; METODOS ESTADISTICOS; NUMERATION CELLULAIRE SOMATIQUE; PCR; PCR-RFLP; POLIMORFISMO; POLYMORPHISM; POLYMORPHISME; Ratios; somatic cell; SOMATIC CELL COUNT; STATISTICAL METHODS; VACAS LECHERAS; VACHE LAITIERE; Zoology
• ISSN: 1344-3941; 1740-0929
• DOI: 10.1111/j.1740-0929.2008.00545.x

Holstein Cows (n=702) from 26 dairy herds in the Tama area of Tokyo, Japan were examined for polymorphisms of the BOLA-DRB3 allele using a PCR-RFLP method. Twenty alleles were observed and allelic frequencies ranged from 1% to 20.3%. Nine alleles (DRB3.2*24, *16, *8, *23, *22, *3, *11, *10 and *7 in order) constituted 90.0% of all alleles. Somatic cell counts (SCC) were used to classify healthy (group 1), mastitis (group 2) and suspected (group 3) cows. Frequencies of DRB3.2*11 and DRB3.2*23 were slightly higher in group 1 than in group 2, whereas, frequencies of DRB3.2*8 and DRB3.2*16 were slightly higher in group 2 than in group 1. However, none of the differences in frequencies between the two groups were statistically significant. For combinations of alleles, frequencies of DRB3.2*8/*23 (P0.1) and DRB3.2*16/*24 (P0.05) were significantly higher in group 2 than in group 1, and their odds ratios were 2.1, 2.5, respectively. However, there were no significant differences between genotypes in their effects for SCC. On the other hand, frequency of DRB3.2*23/*23 including combinations of DRB3.2*23 with minor alleles was significantly higher in group 1 than in group 2 (P0.01 ), and the odds ratio was 0.3. Therefore, it was considered that mastitis resistance or susceptibility of cows may vary with the combination of BoLA-DRB3 alleles.