Fungal transformation of Colletotrichum coccodes with bacterial oahA to suppress defenses of Abutilon theophrasti

Abutilon theophrasti (Malvaceae) is a widely distributed weed that infests a variety of agricultural crops. Cotton is in the same botanical family, precluding the use of selective chemical herbicides. An Abutilon-specific pathovar of Colletotrichum coccodes has been considered as a potential bioherb...

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Bibliographic Details
Published in:Crop protection 2009-09, Vol.28 (9), p.749-755
Main Authors: Meir, Sagit, Larroche, Clemence, Al-Ahmad, Hani, Gressel, Jonathan
Format: Article
Language:English
Subjects:
Abutilon theophrasti
Acid production
Biocontrol
biological control
biological control agents
broadleaf weeds
calcium
Callose
chelating agents
Colletotrichum coccodes
genetic transformation
host plants
hydrolases
Hypervirulence
microbial genetics
mycoherbicides
noxious weeds
oahA gene
Oxalate
oxalic acid
Oxaloacetate acetylhydrolase
pathotypes
resistance mechanisms
virulence
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Summary:Abutilon theophrasti (Malvaceae) is a widely distributed weed that infests a variety of agricultural crops. Cotton is in the same botanical family, precluding the use of selective chemical herbicides. An Abutilon-specific pathovar of Colletotrichum coccodes has been considered as a potential bioherbicide for the selective control of A. theophrasti. An evolutionary balance between microorganism and weed maintains a relatively low virulence of the microorganism, such that it does not destroy its food supply by rapidly killing its host. We overexpressed the oahA (oxaloacetate acetylhydrolase) gene in C. coccodes to increase oxalate production, as oxalate inhibits plant-defensive callose synthase by chelating calcium, an obligate co-factor of this enzyme. C. coccodes oahA transformants acidified the surrounding medium, an indication of acid production by hydrolysis of oxaloacetate. Less callose was seen visually around lesions caused by C. coccodes oahA transformants than the wild type in preliminary experiments, but this was not biochemically quantifiable. Further optimization might be obtained through gene stacking, or the addition of multiple virulence genes to this potential biocontrol agent.
ISSN:0261-2194
1873-6904
DOI:10.1016/j.cropro.2009.04.006