Oligomerization of the potato virus X 25-kD movement protein

A 25-kD movement protein (25K protein) encoded by the first gene of the potexvirus Potato virus X triple gene block of transport genes is essential for the viral movement in infected plants. The 25K protein belongs to superfamily 1 of NTPase/helicases and exhibits in vitro RNA helicase, Mg^sup 2+^-d...

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Bibliographic Details
Published in:Biochemistry (Moscow) 2008-01, Vol.73 (1), p.50-55
Main Authors: Leshchiner, A D, Minina, E A, Rakitina, D V, Vishnichenko, V K, Solovyev, A G, Morozov, S Yu, Kalinina, N O
Format: Article
Language:English
Subjects:
Biochemistry
Genetics
Potato virus X
Potatoes
Potexvirus
Proteins
Ribonucleic acid
RNA
Viruses
Yeasts
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Summary:A 25-kD movement protein (25K protein) encoded by the first gene of the potexvirus Potato virus X triple gene block of transport genes is essential for the viral movement in infected plants. The 25K protein belongs to superfamily 1 of NTPase/helicases and exhibits in vitro RNA helicase, Mg^sup 2+^-dependent NTPase, and RNA-binding activities. In the present work, the ability of 25K protein for homologous interactions was studied using the yeast two-hybrid system, protein chemical cross-linking in the presence of glutaraldehyde, far-Western blotting, and ultracentrifugation in sucrose density gradients. The 25K protein was shown to form homodimers and homooligomers. Sites of homologous protein-protein interactions were found in both the N- and C-terminal portions of the protein. [PUBLICATION ABSTRACT]
ISSN:0006-2979
1608-3040
0320-9725
DOI:10.1007/s10541-008-1007-z