Application of Tissue Microarrays to Study the Influence of Dexamethasone on NF-κB Expression of Pancreas in Rat with Severe Acute Pancreatitis

To discuss the influence of dexamethasone on NF-κB expression of pancreas in rat with severe acute pancreatitis (SAP). Ninety rat SAP models were divided into the model group and dexamethasone treatment group with 45 rats in each group; another healthy 45 rats were selected to be the sham operation...

Full description

Saved in:
Bibliographic Details
Published in:Digestive diseases and sciences 2008-02, Vol.53 (2), p.571-580
Main Authors: Zhang, Xi Ping, Zhang, Ling, Xu, Hong Miao, Xu, Yan Ping, Cheng, Qi Hui, Wang, Jian Mei, Shen, Hai Ping
Format: Article
Language:English
Subjects:
Biochemistry
Biological and medical sciences
Bones, joints and connective tissue. Antiinflammatory agents
Gastroenterology
Gastroenterology. Liver. Pancreas. Abdomen
Hepatology
Liver. Biliary tract. Portal circulation. Exocrine pancreas
Medical sciences
Medicine
Medicine & Public Health
Oncology
Original Paper
Other diseases. Semiology
Pharmacology. Drug treatments
Transplant Surgery
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To discuss the influence of dexamethasone on NF-κB expression of pancreas in rat with severe acute pancreatitis (SAP). Ninety rat SAP models were divided into the model group and dexamethasone treatment group with 45 rats in each group; another healthy 45 rats were selected to be the sham operation group. The groups were divided into the 3, 6 and 12 h group with 15 rats in each group. The survivals, pancreas pathological changes were observed 3, 6 and 12 h after operation. The changes in expression levels of NF-κB protein of pancreas tissue microarray were observed. The treatment group was significantly lower than the model group at 3 and 6 h (P < 0.05) and than the model group at 12 h in pancreas pathological scores (P < 0.01). The expression level of NF-κB protein of pancreas head of the treatment group was significantly less than that of the model group at 3 h (P < 0.01). The alleviation of pancreatic tissue injury by dexamethasone during SAP might be closely related to its role in inhibiting NF-κB expression and regulating cytokines. The advantages of tissue microarrays in pancreatitis pathological examination include time and energy savings, high efficiency and representative results.
ISSN:0163-2116
1573-2568
DOI:10.1007/s10620-007-9867-4