LC/MS Studies on Characterization and Determination of N,N′-Ethylenebisdithiocarbamate Fungicides in Environmental Water Samples

• Published in: Analytical Sciences 2002, Vol.18(4), pp.441-444
• Main Authors: HANADA, Yoshifumi, TANIZAKI, Teizi, KOGA, Minoru, SHIRAISHI, Hiroaki, SOMA, Mitsuyuki
• Format: Article
• Language: English
• Subjects: Analytical chemistry; Applied sciences; Calibration; Chemistry; Chromatographic methods and physical methods associated with chromatography; Continental surface waters; Exact sciences and technology; Fresh Water - analysis; Fungicides, Industrial - analysis; Gas Chromatography-Mass Spectrometry - methods; Maneb - analysis; Natural water pollution; Other chromatographic methods; Pollution; Spectrometry, Mass, Electrospray Ionization - methods; Water Supply - analysis; Water treatment and pollution; Zineb - analysis
• ISSN: 0910-6340; 1348-2246
• DOI: 10.2116/analsci.18.441

Liquid chromatography/mass spectrometry (LC/MS) has been applied to analyze N,N′-ethylenebisdithiocarbamate fungicides (EBDCs: manzeb, maneb and zineb) in environmental water samples. The EBDCs that are zinc and/or manganese salts are transformed into readily water-soluble sodium salts by adding an alkaline EDTA solution. The N,N′-ethylenebisdithiocarbamate anion is extracted into chloroform-hexane (3:1) as an ion pair with tetrabutylammonium, and then S-alkylated with methyl iodide. The extraction and derivatization are carried out at room temperature. The derivatized ethylenebisdithiocarbamic acid dimethyl ester is introduced into an LC/MS equipped with a negative ion electrospray ionization interface. Identification of the compound is performed with the specific quasi-molecular ion, and the quantitative analyses are carried out using the peak areas. The average recoveries and coefficients of variation of EBDCs at sub-ppb level are 79.1% and 29.3% (n = 6), respectively. The limit of detection based on standard deviation of 0.043 μg/L for manzeb is achieved.