rapid technique for screening banana cultivars for resistance to Xanthomonas wilt

The banana Xanthomonas wilt disease (BXW) has threatened the livelihood of millions of farmers in East Africa. Use of resistant varieties is the most cost-effective method of managing this bacterial disease. A reliable and rapid screening method is needed to select resistant banana varieties. An in...

Full description

Saved in:
Bibliographic Details
Published in:European journal of plant pathology 2008-05, Vol.121 (1), p.9-19
Main Authors: Tripathi, Leena, Odipio, John, Tripathi, Jaindra Nath, Tusiime, Geoffrey
Format: Article
Language:English
Subjects:
Agriculture
Bacterial diseases
Bacterial plant pathogens
Banana cultivars
Bananas
Biological and medical sciences
Biomedical and Life Sciences
Cultivars
Ecology
Fruits
Full Research Paper
Fundamental and applied biological sciences. Psychology
Life Sciences
Musa
Pathogens
Phytopathology. Animal pests. Plant and forest protection
Plant diseases
Plant Pathology
Plant resistance
Plant Sciences
Screening method
Xanthomonas
Xanthomonas campestris
Xanthomonas wilt
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The banana Xanthomonas wilt disease (BXW) has threatened the livelihood of millions of farmers in East Africa. Use of resistant varieties is the most cost-effective method of managing this bacterial disease. A reliable and rapid screening method is needed to select resistant banana varieties. An in vitro screening method was developed for early evaluation of Xanthomonas wilt resistance using small tissue culture-grown plantlets. Eight cultivars of banana were screened with sixteen isolates of Xanthomonas campestris pv. musacearum using this method. There were significant differences (P < 0.0001) in susceptibility among the various banana cultivars tested, whereas no significant difference (P = 0.92) in pathogenicity was observed between the pathogen isolates. The cv. Pisang Awak (Kayinja) was found to be highly susceptible and Musa balbisiana resistant. Nakitembe was found to be moderately resistant while cvs Mpologoma, Mbwazirume, Sukali Ndiizi, FHIA-17 and FHIA-25 were susceptible. The susceptibility of these cultivars was further tested in vivo by artificial inoculation of potted plants with similar results. This study shows that an in vitro screening test can serve as a convenient, cheap and rapid screening technique to discriminate BXW-resistant from BXW-susceptible banana cultivars.
ISSN:0929-1873
1573-8469
DOI:10.1007/s10658-007-9235-4