miR-335-5p targeting ICAM-1 inhibits invasion and metastasis of thyroid cancer cells

• Published in: Biomedicine & pharmacotherapy 2018-10, Vol.106, p.983-990
• Main Authors: Luo, Li, Xia, Li, Zha, Binshan, Zuo, Chunlin, Deng, Datong, Chen, Mingwei, Hu, Linhong, He, Yong, Dai, Fang, Wu, Jing, Wang, Changjiang, Wang, Youmin, Zhang, Qiu
• Format: Article
• Language: English
• Subjects: 3' Untranslated Regions; Apoptosis; Binding Sites; Carcinoma, Papillary - genetics; Carcinoma, Papillary - metabolism; Carcinoma, Papillary - secondary; Cell Line, Tumor; Cell Movement; Cell Proliferation; Gene Expression Regulation, Neoplastic; Humans; ICAM-1; Intercellular Adhesion Molecule-1 - genetics; Intercellular Adhesion Molecule-1 - metabolism; MicroRNAs - genetics; MicroRNAs - metabolism; miR-335-5p; miRNAs; Neoplasm Invasiveness; Signal Transduction; Thyroid cancer; Thyroid Cancer, Papillary; Thyroid Neoplasms - genetics; Thyroid Neoplasms - metabolism; Thyroid Neoplasms - pathology; Time Factors
• ISSN: 0753-3322; 1950-6007
• DOI: 10.1016/j.biopha.2018.07.046

•miR-335-5p could target 3′UTR of ICAM-1 and inhibit its expression.•miR-335-5p was anti-oncogene in thyroid cancer.•miR-335-5p may be a potential small molecule drug for thyroid cancer. miRNAs is a kind of noncoding small RNAs with negative regulation function. Some miRNAs play a crucial role in the growth of tumor cells. In this study, we analyzed the role of miR-335-5p and its target gene intercellular adhesion molecule 1 (ICAM-1) in thyroid cancer. Real-time polymerase chain reaction (PCR) results showed that the expression level of ICAM-1 in cancer tissues was higher than that in their adjacent tissues. The expression level of ICAM-1 in papillary thyroid carcinoma was also significantly higher than that in other types of tumors. However, the expression of miR-335-5p is opposite to that of ICAM-1. In human thyroid cancer cell lines TPC-1, FTC-133, TT and human thyroid follicular cell line Nthyori 3-1, the expression level of ICAM-1 in TPC-1 was significantly higher than that of other cells, while the expression level of miR-335-5p in TPC-1 was significantly lower than that of other cells. When ICAM-1 expression was downregulated and miR-335-5p expression was upregulated in TPC-1 cells, ICAM-1 expression was upregulated and miR-335-5p expression was downregulated in FTC-133 cells, we found that ICAM-1 could promote the proliferation of thyroid cancer cells, while miR-335-5p could inhibit the proliferation of thyroid cancer cells. miR-335-5p could combine with 3′UTR of ICAM-1 by bioinformatics prediction. Luciferase reporter gene analysis and Western blotting detection further confirmed that miR-335-5p could target ICAM-1 and inhibit its expression. The expression level of miR-335-5p was downregulated, while the expression level of ICAM-1 was upregulated in thyroid cancer. This study will help us better understand the pathogenesis of thyroid cancer and provide new insights into the treatment of this disease.