Measurement of apixaban, dabigatran, edoxaban and rivaroxaban in human plasma using automated online solid-phase extraction combined with ultra-performance liquid chromatography-tandem mass spectrometry and its comparison with coagulation assays

Measurement of direct oral anticoagulants (DOACs) concentration in patient blood is essential in special clinical circumstances. We developed a fast, selective and sensitive method for simultaneous measurement of DOACs in human plasma consisting of an automated online solid-phase extraction method c...

Full description

Saved in:
Bibliographic Details
Published in:Clinica chimica acta 2018-11, Vol.486, p.347-356
Main Authors: Kuhn, Joachim, Gripp, Tatjana, Flieder, Tobias, Hammerschmidt, Andreas, Hendig, Doris, Faust, Isabel, Knabbe, Cornelius, Birschmann, Ingvild
Format: Article
Language:English
Subjects:
Anticoagulants
Anticoagulants - chemistry
Apixaban
Automation
Calibration
Chromatography, High Pressure Liquid
Dabigatran
Dabigatran - blood
Edoxaban
Humans
Pyrazoles - blood
Pyridines - blood
Pyridones - blood
Rivaroxaban
Rivaroxaban - blood
Solid Phase Extraction
Tandem Mass Spectrometry
Thiazoles - blood
Ultra-performance liquid chromatography-tandem mass spectrometry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Measurement of direct oral anticoagulants (DOACs) concentration in patient blood is essential in special clinical circumstances. We developed a fast, selective and sensitive method for simultaneous measurement of DOACs in human plasma consisting of an automated online solid-phase extraction method coupled with ultra-performance liquid chromatography electrospray ionization-tandem mass spectrometry (online SPE-UPLC-MS/MS). The calibration curves of all DOACs were linear over the working range (apixaban: 0.25–760 μg/L, r > 0.99; dabigatran: 0.5–900 μg/L, r > 0.99; edoxaban: 0.6–800 μg/L, r > 0.99; rivaroxaban: 0.5–900 μg/L, r > 0.99). Limits of detection in the plasma matrix were 
ISSN:0009-8981
1873-3492
DOI:10.1016/j.cca.2018.08.017