Clinical application of Clostridium botulinum type A neurotoxin purified by a simple procedure for patients with urinary incontinence caused by refractory detrusor overactivity

Type A neurotoxin of Clostridium botulinum was purified by a simple procedure using a lactose gel column. This procedure was previously reported for type B neurotoxin. Hemagglutinin-positive toxins (19S and 16S) were bound to the column under acid conditions, and the neurotoxin alone was dissociated...

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Bibliographic Details
Published in:Toxicon (Oxford) 2008-06, Vol.51, p.41-41
Main Authors: Keiji, Oguma, Jae-Chul, Lee, Teruyuki, Yokoyama, Yumiko, Yamamoto, Yoshihiko, Sakaguchi, Shaobo, Ma, Hideyuki, Arimitsu
Format: Article
Language:English
Subjects:
Clostridium botulinum
Detrusor overactivity
Neurotoxin
Purification
Therapy
Urinary incontinence
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Summary:Type A neurotoxin of Clostridium botulinum was purified by a simple procedure using a lactose gel column. This procedure was previously reported for type B neurotoxin. Hemagglutinin-positive toxins (19S and 16S) were bound to the column under acid conditions, and the neurotoxin alone was dissociated from these hemagglutinin-positive toxins by changing the pH of the column to an alkaline condition. The toxicity of this purified toxin preparation was retained for at least 1 year at ?30 °C by supplementing it with either 0.1% albumin or 0.05% albumin plus 1% trehalose. This preparation was used to treat 18 patients with urinary incontinence caused by refractory idiopathic and neurogenic detrusor overactivity; 16 of the patients showed excellent improvement. Improvements started within 1 week after injection in most cases and lasted 3–12 months.
ISSN:0041-0101
1879-3150
DOI:10.1016/j.toxicon.2008.04.122