Comparison of numerical change of epidermal growth factor receptor gene among pre- and postradiation glioma, and gliosis, and its clinical use

Surgery with following chemoradiotherapy is the mainstream glioma treatment. In the course of postradiation events, however, it is sometimes difficult for neurosurgeons, radiologists, and pathologists to discriminate tumor recurrence from radiation necrosis. The epidermal growth factor receptor (EGF...

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Bibliographic Details
Published in:Brain tumor pathology 2007-05, Vol.24 (1), p.15-18
Main Authors: Okada, Yoshifumi, Ohno, Chihiro, Ueki, Keisuke, Ogino, Masahiro, Kawamoto, Shunsuke, Kim, Phyo
Format: Article
Language:English
Subjects:
Adult
Aged
Brain cancer
Brain Neoplasms - genetics
Brain Neoplasms - pathology
Brain Neoplasms - radiotherapy
Epidermal growth factor
Female
Genes, erbB-1
Glioma
Glioma - genetics
Glioma - pathology
Glioma - radiotherapy
Gliosis - etiology
Gliosis - genetics
Gliosis - pathology
Humans
In Situ Hybridization, Fluorescence
Male
Middle Aged
Necrosis
Radiation
Radiation Injuries - genetics
Radiation Injuries - pathology
Radiotherapy - adverse effects
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Summary:Surgery with following chemoradiotherapy is the mainstream glioma treatment. In the course of postradiation events, however, it is sometimes difficult for neurosurgeons, radiologists, and pathologists to discriminate tumor recurrence from radiation necrosis. The epidermal growth factor receptor (EGFR) gene, on chromosome 7, is known to gain in copy number frequently in high-grade gliomas. The authors applied the fluorescence in situ hybridization (FISH) method to observe the gene's numerical status in pre- and postradiation glioma samples to elucidate whether this technique is useful in the discrimination of glioma recurrence from radiation necrosis. When 15 postradiation glioma samples and 4 postradiation nonglioma samples were tested, all the recurrent glioma tissue harbored numerical aberrations of the gene, whereas no abnormality could be observed in necrosis or in nonglioma gliosis. FISH could even prove a residual glioma cell in a gliotic tissue taken by needle biopsy after gamma-knife radiosurgery, which had been executed on a supposed metastatic brain tumor. FISH is considered to be of help in accurate diagnosis, especially when the usual histopathological diagnosis is difficult because of radiation effects or small sample size.
ISSN:1433-7398
1861-387X
DOI:10.1007/s10014-007-0213-5