Stable expression of foot-and-mouth disease virus protein VP1 fused with cholera toxin B subunit in the potato (Solanum tuberosum)

• Published in: Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2007-04, Vol.55 (2), p.159-163
• Main Authors: He, Dong-Mei, Qian, Kai-Xian, Shen, Gui-Fang, Li, Yi-Nü, Zhang, Zhi-Fang, Su, Zhong-Liang, Shao, Hong-Bo
• Format: Article
• Language: English
• Subjects: Blotting, Southern; Blotting, Western; Carboxylic Ester Hydrolases - genetics; Cholera Toxin - genetics; Cholera toxin B subunit (CTB); Enzyme-Linked Immunosorbent Assay; Foot-and-mouth disease virus; Foot-and-mouth disease virus (FMDV); Foot-and-Mouth Disease Virus - genetics; Fused gene; Fused proterin; Gene engineering; Gene Expression; Immunogenicity; Patatin promoter; Plant Proteins - genetics; Plants, Genetically Modified; Polymerase Chain Reaction; Promoter Regions, Genetic - genetics; Protein fusion; Recombinant Fusion Proteins - genetics; Solanum tuberosum; Solanum tuberosum - genetics; Solanum tuberosum - metabolism
• ISSN: 0927-7765; 1873-4367
• DOI: 10.1016/j.colsurfb.2006.11.043

The expression vector, pBI121CTBVP1, containing the fusion of the foot and mouth disease virus (FMDV) VP1 gene and the cholera toxin B subunit (CTB) gene was constructed by fused PCR and transferred into potato (Solanum tuberosum L.) by Agrobacterium-mediated transformation. Transformed plants were obtained by selecting on kanamycin-resistant medium strictly and regenerated. The transgenic plantlets were identified by PCR, Southern-blot and the production of fused protein was confirmed and quantified by Western-blot and ELISA assays. The results showed that the fused genes were expressed stablely under the control of specific-tuber patatin promoter. The expressed fused proteins have a certain degree of immunogenicity.