Aflatoxin B1 impairs spermatogenesis: An experimental study for crosslink between oxidative stress and mitochondria‐dependent apoptosis

The present experimental study was carried out to investigate the crosslink between aflatoxin B1 (AFB1)‐induced oxidative stress and mitochondria‐dependent apoptosis in testicles. For this purpose, 24 mature male Swiss albino mice were randomly divided into control and test groups. The AFB1 was diss...

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Bibliographic Details
Published in:Environmental toxicology 2018-11, Vol.33 (11), p.1204-1213
Main Authors: Yasin, Malekzadeh, Mazdak, Razi, Mino, Ilkhanipour
Format: Article
Language:English
Subjects:
aflatoxin
Aflatoxin B1
Aflatoxin B1 - toxicity
Albinism
Animals
Apoptosis
Apoptosis - drug effects
BAX protein
Body weight
Caspase
Corn oil
Crosslinking
DNA
Epithelium
Ethanol
Fuel consumption
Gene expression
Immunohistochemistry
In Situ Nick-End Labeling
intrinsic apoptosis
Male
Mice
Mitochondria
Mitochondria - drug effects
Mitochondria - physiology
Nucleotide sequence
Organ Size - drug effects
Oxidative stress
Oxidative Stress - drug effects
p53 Protein
PCR
Proteins
Reverse transcription
Spermatogenesis
Spermatogenesis - drug effects
Spermiogenesis
Testis - drug effects
Testis - growth & development
Testis - metabolism
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Summary:The present experimental study was carried out to investigate the crosslink between aflatoxin B1 (AFB1)‐induced oxidative stress and mitochondria‐dependent apoptosis in testicles. For this purpose, 24 mature male Swiss albino mice were randomly divided into control and test groups. The AFB1 was dissolved in corn oil and ethanol (95:5, v/v) vehicle. The animals in test group subdivided into three groups, which received the AFB1 at a daily dose of 20 μg/kg body weight, through intraperitoneal (i.p.) route, for 7, 14, and 21 days. The mice in the control group received the vehicle alone for 21 days. The expression of Bcl‐2, Bax, p53, and caspase‐3 at both mRNA and protein levels were analyzed by using reverse transcription PCR (RT‐PCR) and immunohistochemistry, respectively. Moreover, the mitochondrial content of germinal epithelium, tubular differentiation (TDI), and spermiogenesis (SPI) indices was analyzed. Finally, the apoptosis was assessed by using TUNEL staining. Observations revealed that the AFB1 remarkably (P 
ISSN:1520-4081
1522-7278
DOI:10.1002/tox.22627