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Development of reference material with assigned value for human T‐cell leukemia virus type 1 quantitative PCR in Japan
ABSTRACT Quantitative PCR (qPCR) of human T‐cell leukemia virus type 1 (HTLV‐1) provirus is used for HTLV‐1 testing and for assessment of risk of HTLV‐1‐related diseases. In this study, a reference material was developed for standardizing HTLV‐1 qPCR. Freeze‐dried TL‐Om1 cells diluted with Jurkat ce...
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Published in: | Microbiology and immunology 2018-10, Vol.62 (10), p.673-676 |
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Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | ABSTRACT
Quantitative PCR (qPCR) of human T‐cell leukemia virus type 1 (HTLV‐1) provirus is used for HTLV‐1 testing and for assessment of risk of HTLV‐1‐related diseases. In this study, a reference material was developed for standardizing HTLV‐1 qPCR. Freeze‐dried TL‐Om1 cells diluted with Jurkat cells were prepared and an assigned value for proviral load (PVL) of 2.71 copies/100 cells was determined by digital PCR. Nine Japanese laboratories using their own methods evaluated the PVLs of this reference material as 1.08–3.49 copies/100 cells. The maximum difference between laboratories was 3.2‐fold. Correcting measured PVLs by using a formula incorporating the assigned value of this reference material should minimize such discrepancies. |
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ISSN: | 0385-5600 1348-0421 |
DOI: | 10.1111/1348-0421.12644 |