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Development of reference material with assigned value for human T‐cell leukemia virus type 1 quantitative PCR in Japan

ABSTRACT Quantitative PCR (qPCR) of human T‐cell leukemia virus type 1 (HTLV‐1) provirus is used for HTLV‐1 testing and for assessment of risk of HTLV‐1‐related diseases. In this study, a reference material was developed for standardizing HTLV‐1 qPCR. Freeze‐dried TL‐Om1 cells diluted with Jurkat ce...

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Published in:Microbiology and immunology 2018-10, Vol.62 (10), p.673-676
Main Authors: Kuramitsu, Madoka, Okuma, Kazu, Nakashima, Makoto, Sato, Tomoo, Sasaki, Daisuke, Hasegawa, Hiroo, Umeki, Kazumi, Kubota, Ryuji, Sasada, Keiko, Sobata, Rieko, Matsumoto, Chieko, Kaneko, Noriaki, Tezuka, Kenta, Matsuoka, Sahoko, Utsunomiya, Atae, Koh, Ki‐Ryang, Ogata, Masao, Ishitsuka, Kenji, Taki, Mai, Nosaka, Kisato, Uchimaru, Kaoru, Iwanaga, Masako, Sagara, Yasuko, Yamano, Yoshihisa, Okayama, Akihiko, Miura, Kiyonori, Satake, Masahiro, Saito, Shigeru, Watanabe, Toshiki, Hamaguchi, Isao
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Language:English
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Summary:ABSTRACT Quantitative PCR (qPCR) of human T‐cell leukemia virus type 1 (HTLV‐1) provirus is used for HTLV‐1 testing and for assessment of risk of HTLV‐1‐related diseases. In this study, a reference material was developed for standardizing HTLV‐1 qPCR. Freeze‐dried TL‐Om1 cells diluted with Jurkat cells were prepared and an assigned value for proviral load (PVL) of 2.71 copies/100 cells was determined by digital PCR. Nine Japanese laboratories using their own methods evaluated the PVLs of this reference material as 1.08–3.49 copies/100 cells. The maximum difference between laboratories was 3.2‐fold. Correcting measured PVLs by using a formula incorporating the assigned value of this reference material should minimize such discrepancies.
ISSN:0385-5600
1348-0421
DOI:10.1111/1348-0421.12644