Response of the blue shrimp Litopenaeus stylirostris to temperature decrease and hypoxia in relation to molt stage

• Published in: Aquaculture 2005-02, Vol.244 (1), p.315-322
• Main Authors: Mugnier, Chantal, Soyez, Claude
• Format: Article
• Language: English
• Subjects: Animal aquaculture; Animal productions; Aquaculture; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; General aspects; Hypoxia; Litopenaeus stylirostris; Marine; Molt stage; Osmoregulation; Penaeid shrimp; Shellfish; Stress; Temperature
• ISSN: 0044-8486; 1873-5622
• DOI: 10.1016/j.aquaculture.2004.11.010

The effect of different levels of dissolved oxygen (DO) and a decreased temperature combined with severe hypoxia were studied in the blue shrimp Litopenaeus stylirostris in relation to molt stage. In the first experiment, shrimps were submitted to DO concentrations of 5 to 1 mg l −1. Osmoregulatory capacity (OC) was measured in shrimps at molt stage C after 6, 24, or 48 h of exposure. No mortality was recorded but a significant negative effect of DO concentration on hypo-OC was observed for DO concentrations below 3 mg l −1. Osmoregulation improved after 48 h exposure, compared to 6 and 24 h exposure. In a second experiment, temperature was decreased from 28 to 22 °C in 24 h, before a severe hypoxia of 0.5±0.1 mg O 2 l −1 was applied. Mortality and OC were studied in relation to molt stages. Half of the shrimps died after respectively 100 min at 0.5 mg O 2 l −1 at 28 °C and 153 min at 0.4 mg O 2 l −1 at 22°C. Mortality was significantly higher in stages D 2 and B compared to stages C and D 0. Control shrimps in stage D 2 had a lower hypo-OC than shrimps in stages C and D 0 both at 28 and 22 °C. Temperature decrease had no effect on hypo-OC. Hypoxia reduced hypo-OC for all stages, whatever the temperature, but the effect was greater in stage C at 27 °C (68% decrease) compared to 22 °C (49%) and stage D 2 at both temperatures (respectively, 43% and 58% at 27 and 22 °C). Combination of temperature and hypoxia had a significant effect on hypo-OC. Low temperature reduced the effect of hypoxia presumably by slowing down the metabolism. Results are discussed in relation to pond observation.