Combinational Effect of Green Tea, Phytic Acid and Inositol on Bone Mineralization and Mineral Balance in with Azoxymethane-Induced Colon Carcinogenesis Induced Fisher 344 Male Rats

• Published in: Journal of pharmacology & toxicology 2008, Vol.3 (4), p.279-290
• Main Authors: Sunkara, R., ., M.Verghese, ., J. Khatiwada, ., L. Shackelford, ., J. Boateng, ., L.T. Walker, Chawan, C.B.
• Format: Article
• Language: English
• ISSN: 1816-496X
• DOI: 10.3923/jpt.2008.279.290

The aim of the study was to determine the combinational effect of dietary Phytic Acid (PA), Green Tea (GT) and Inositol (I) at 1 and 2% level (in drinking water) on bone mineralization in rats with azoxymethane (AOM)-induced colon carcinogenesis. After one week period of acclimatization, 9 groups of rats (6 rats each) were fed AIN 93G (till 20 week) and later switched to AIN 93 M diets (till 45 weeks age). All rats received AOM s/c at the rate of 16 mg kg super(-1) body weight at 7 and 8 weeks of age. Urine and fecal samples were collected for a 12 day period. Rats were killed by CO sub(2) asphyxiation at 46 week of age and samples (cecum, blood, tibia and femur) were collected and analyzed by ICP for selected minerals (Ca, P, Mg, Fe and Zn). Physical parameters (weight, length, circumference and volume) of tibia and femur were examined. There were no significant differences in apparent absorption, retention and serum concentrations of macro minerals (Ca, P and Mg), although apparent absorption, bone and serum levels of Fe and Zn were significantly lower in 2% combinations. Results of this study showed that combination of treatments at lower levels may be beneficial in reducing the negative effects on bone mineralization.