Expression of prolactin receptors in the duodenum, kidneys and skeletal system during physiological and sulpiride-induced hyperprolactinaemia

Introduction and aim Hyperprolactinaemia in pregnancy leads to mild and reversible changes in the maternal skeletal system, and medicamentous hyperprolactinemia causes more detrimental effects. We conducted an experimental study to evaluate differences between Prlr gene expression in the duodenum, v...

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Bibliographic Details
Published in:Endocrine 2018-12, Vol.62 (3), p.681-691
Main Authors: Radojkovic, Danijela, Pesic, Milica, Radojkovic, Milan, Dimic, Dragan, Vukelic Nikolic, Marija, Jevtovic Stoimenov, Tatjana, Radenkovic, Sasa, Velojic Golubovic, Milena, Radjenovic Petkovic, Tatjana, Antic, Slobodan
Format: Article
Language:English
Subjects:
Animals
Bone and Bones - metabolism
Bone turnover
Calcium (urinary)
Calcium - blood
Calcium absorption
Calcium homeostasis
Diabetes
Duodenum
Duodenum - metabolism
Endocrinology
Excretion
Female
Gene expression
Homeostasis
Humanities and Social Sciences
Hyperprolactinemia
Hyperprolactinemia - chemically induced
Hyperprolactinemia - metabolism
Internal Medicine
Intestine
Kidney - metabolism
Kidneys
Medicine
Medicine & Public Health
multidisciplinary
Original Article
Osteocalcin
Osteocalcin - blood
Parathyroid
Parathyroid hormone
Parathyroid Hormone - blood
Phosphorus
Phosphorus - blood
Physiology
Pregnancy
Procollagen
Prolactin
Prolactin receptors
Rats
Rats, Wistar
Receptors, Prolactin - genetics
Receptors, Prolactin - metabolism
Rodents
Science
Spine
Sulpiride
Vertebrae
Vitamin D
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Summary:Introduction and aim Hyperprolactinaemia in pregnancy leads to mild and reversible changes in the maternal skeletal system, and medicamentous hyperprolactinemia causes more detrimental effects. We conducted an experimental study to evaluate differences between Prlr gene expression in the duodenum, vertebrae and kidneys during physiological and medicamentous hyperprolactinaemia, which could influence calcium homeostasis. Methods Experimental animals (18 weeks old, Wistar female rats) were divided as follows: group P (nine rats that were 3 weeks pregnant), group M (ten rats that were intramuscularly administrated sulpiride (10 mg/kg) twice daily for 3 weeks), and the control group (C, ten age-matched nulliparous rats, 18-week-old). Laboratory investigations included measurements of serum ionized calcium, phosphorus, urinary calcium and phosphorus excretion, osteocalcin (OC), serum procollagen type 1 N-terminal propeptide (P1NP), vitamin D, parathyroid hormone (PTH) and prolactin (PRL). Relative quantification of gene expression for prolactin receptors in the duodenum, vertebrae and kidneys was determined using real-time PCR. Results Expression of the Prlr gene was significantly higher in the duodenum ( p  
ISSN:1355-008X
1559-0100
DOI:10.1007/s12020-018-1730-1