Improved translation efficiency in chrysanthemum and torenia with a translational enhancer derived from the tobacco alcohol dehydrogenase gene

• Published in: Plant Biotechnology 2008/03/01, Vol.25(1), pp.69-75
• Main Authors: Aida, Ryutaro, Narumi, Takako, Ohtsubo, Norihiro, Yamaguchi, Hiroyasu, Kato, Ko, Shinmyo, Atsuhiko, Shibata, Michio
• Format: Article
• Language: English
• Subjects: Cauliflower mosaic virus; Chrysanthemum; Chrysanthemum morifolium; molecular breeding; ornamental plants; Torenia fournieri; translational enhancer
• ISSN: 1342-4580; 1347-6114
• DOI: 10.5511/plantbiotechnology.25.69

We investigated the efficiency of the 5′-untranslated region (UTR) of the tobacco alcohol dehydrogenase gene (NtADH-5′UTR) as a translational enhancer in chrysanthemum (Chrysanthemum morifolium) and torenia (Torenia fournieri). Three constructs were introduced: control, “ADH−,” cauliflower mosaic virus 35S RNA (CaMV 35S) promoter::β-glucuronidase (GUS); NtADH-5′UTR with a spacer, “ADH+S,” CaMV 35S promoter::NtADH-5′UTR::25-bp spacer::GUS; and NtADH-5′UTR with no spacer, “ADH+,” CaMV 35S promoter::NtADH-5′UTR::GUS. The highest GUS activity in ADH+S and ADH+ for chrysanthemum was about 45 and 190 times, respectively, and for torenia was 12 and 22 times, respectively, than that for the ADH− plants. NtADH-5′UTR enhanced translational efficiency in both species. With the lowest translational efficiency set to 1, the relative translational efficiencies were 1 to 15 (ADH−), 47 to 568 (ADH+S), and 267 to 1360 (ADH+) for chrysanthemum, and 1 to 3 (ADH−), 47 to 114 (ADH+S), and 85 to 226 (ADH+) for torenia. NtADH-5′UTR would facilitate practical breeding and fundamental genetic research in chrysanthemum and torenia.