Response of bacteriorhodopsin thin films to ammonia

The optical response of optical fibres end-coated with bacteriorhodopsin-containing films to ammonia vapour was investigated. Both static absorptance changes and changes in the dynamic parameters of the actinic light-induced photocycle were determined. Simple optical adsorption measurements can be u...

Full description

Saved in:
Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 2008-01, Vol.129 (1), p.473-480
Main Authors: Korposh, S.O., Sharkan, Y.P., Ramsden, J.J.
Format: Article
Language:English
Subjects:
Bacteria
Bacteriorhodopsin
Chemical additive
Fibre-optic sensor
Photocycle
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The optical response of optical fibres end-coated with bacteriorhodopsin-containing films to ammonia vapour was investigated. Both static absorptance changes and changes in the dynamic parameters of the actinic light-induced photocycle were determined. Simple optical adsorption measurements can be used to detect ammonia with a detection limit of 5 ppm and a dynamic range of 10–10,000 ppm, over which the response is logarithmic. By analysing the modulation of the photocycle response by ammonia, the limit of detection can be decreased to 0.9 ppm. The incorporation of chemicals with an amine functionality, such as triethanolamine, in the film suppresses the ammonia response, and is therefore useful for referencing.
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2007.08.024