COX expression and PGE2 and PGD2 production in experimental acute and chronic gastric lesions

Prostaglandin E2 and D2 (PGE2 and PGD2) production and cyclooxygenase-2 (COX-2) expression during the resolution of acute and chronic gastric inflammatory lesions in Wistar rats have been investigated. Differences between ibuprofen, nonselective COX inhibitor, and rofecoxib, specific COX-2 inhibitor...

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Published in:International immunopharmacology 2005-02, Vol.5 (2), p.369-379
Main Authors: MOTILVA, Virginia, ALARCON DE LA LASTRA, Catalina, BRUSEGHINI, Leo, HERRERIAS, Juan Manuel, SANCHEZ-FIDALGO, Susana
Format: Article
Language:English
Subjects:
Biological and medical sciences
Medical sciences
Pharmacology. Drug treatments
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Summary:Prostaglandin E2 and D2 (PGE2 and PGD2) production and cyclooxygenase-2 (COX-2) expression during the resolution of acute and chronic gastric inflammatory lesions in Wistar rats have been investigated. Differences between ibuprofen, nonselective COX inhibitor, and rofecoxib, specific COX-2 inhibitor, on the development of the induced responses were also analysed. In an acute model, by instillation of HCL, the greatest injury was observed early with a rapid and progressive restoration. Maximal up-regulation of COX-2 protein was detected at 6 h and was accompanied by increase of PGE2 synthesis but not PGD2. Both drugs stimulated COX-2 expression in accordance to their capacity of inhibiting this enzymatic activity, driving to delay in the healing. In a chronic model, by acetic acid-induced gastric ulcers, COX-2 was expressed at 7 days and was also associated with PGE2 increase. Ibuprofen and rofecoxib also augmented COX-2 protein and inhibited PGE2 levels. However, PGD2 production was augmented when none signal of COX-2 protein could be detected. Together, this study confirms the role played by COX-2 enzyme in the resolution of acute and chronic gastric inflammatory process, PGE2 being the principal product. The antiinflammatory effect of nonsteroidal antiinflammatory drugs (NSAIDs) could be mediated not only through the inhibition of COX activity but also through the induction of antiinflammatory PGs production-such as PGD2-although further studies would be needed to clarify the mechanisms of this activity and the possible implicated processes.
ISSN:1567-5769
1878-1705
DOI:10.1016/j.intimp.2004.10.005