(OP 319) Test and Validation of a Bioreactor for the Stimulation of Engineered Cartilage Constructs with Combined Regimens of Hydrostatic Pressure and Interstitial Perfusion

During joint physiological loading in vivo, the complex mechanical stimuli which act simultaneously on chondrocytes include hydrodynamic shear stress, cell deformation and increased interstitial pressure. Bioreactor systems represent valuable instruments to investigate the roles of several culture p...

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Bibliographic Details
Published in:Tissue engineering. Part A 2008-05, Vol.14 (5), p.797-797
Main Authors: Moretti, M, Freed, LE, Padera, R F, Lagana, K, Raimondi, M T
Format: Article
Language:English
Online Access:Get full text
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Summary:During joint physiological loading in vivo, the complex mechanical stimuli which act simultaneously on chondrocytes include hydrodynamic shear stress, cell deformation and increased interstitial pressure. Bioreactor systems represent valuable instruments to investigate the roles of several culture parameters, although single rather than combined stimuli have often been studied. The present study tested and validated a bioreactor developed for culturing tissue engineered cartilage in the presence of different levels of hydrodynamic shear stresses and hydrostatic pressures. Primary bovine chondrocytes were seeded on non-woven scaffolds. Constructs were cultured for 5 days in Petri dishes and then for 5 additional days as follows: Group-1 with interstitial perfusion (0.4 mL/min) combined with hydrostatic pressure (10MPa, 1 Hz for 4 h/day), Group-2 with interstitial perfusion (0.4 mL/min) without hydrostatic pressure, and Group-3 in static Petri dishes. A total of 112 cartilage constructs were studied in four independent experiments. Cell viability, assessed as MTT/DNA, was significantly higher (p
ISSN:1937-3341
1937-335X