Super-oxidized solution inhibits IgE-antigen-induced degranulation and cytokine release in mast cells

• Published in: International immunopharmacology 2007-08, Vol.7 (8), p.1013-1024
• Main Authors: Medina-Tamayo, J., Sánchez-Miranda, E., Balleza-Tapia, H., Ambriz, X., Cid, M.E., González-Espinosa, D., Gutiérrez, A.A., González-Espinosa, C.
• Format: Article
• Language: English
• Subjects: Allergy; Animals; beta-N-Acetylhexosaminidases - metabolism; Biological and medical sciences; Blotting, Western; Bone Marrow Cells - cytology; Bone Marrow Cells - drug effects; Bone Marrow Cells - metabolism; Calcium - metabolism; Cell Degranulation - drug effects; Cell Degranulation - immunology; Cell Survival - drug effects; Cytokine production; Cytokines - genetics; Cytokines - metabolism; Degranulation; Disinfectants - toxicity; Enzyme-Linked Immunosorbent Assay - methods; Hydrogen Peroxide - toxicity; IgE receptor; Immunoglobulin E - immunology; Mast cell; Mast Cells - drug effects; Mast Cells - metabolism; Mast Cells - physiology; Medical sciences; Mice; Mice, Inbred Strains; Mitogen-Activated Protein Kinases - metabolism; NFATC Transcription Factors - metabolism; Pharmacology. Drug treatments; Phosphorylation - drug effects; Receptors, IgE - metabolism; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - genetics; RNA, Messenger - metabolism; Sodium Hypochlorite - toxicity; Super-oxidized solution; Tyrosine - metabolism
• ISSN: 1567-5769; 1878-1705
• DOI: 10.1016/j.intimp.2007.03.005

Activation of the high affinity IgE receptor (FcεRI) through IgE-antigen complexes induces mast cell degranulation, synthesis of lipid mediators and cytokine production. These effects are involved in Type I hypersensitivity reactions and controlling them has been the main objective of many anti-allergic therapies. Here we report that pretreatment of murine bone marrow derived mast cells (BMMC) with super-oxidized solution (SOS) inhibits FcεRI dependent-β hexosaminidase and cytokine release. This effect is exerted without altering total protein tyrosine phosphorylation, MAPK activation, cytokine mRNA accumulation or calcium mobilization after FcεRI triggering. Our data suggest that this neutral pH-SOS acts like a mast cell-membrane stabilizer inhibiting the cell machinery for granule secretion without altering the signal transduction pathways induced by IgE-antigen receptor crosslinking.