Preventive effect of apigenin against isoproterenol‐induced apoptosis in cardiomyoblasts

• Published in: Journal of biochemical and molecular toxicology 2018-11, Vol.32 (11), p.e22213-N/A
• Main Authors: Thangaiyan, Radhiga, Robert, Beaulah Mary, Arjunan, Sundaresan, Govindasamy, Kanimozhi, Nagarajan, Rajendra Prasad
• Format: Article
• Language: English
• Subjects: Animals; Anti-Inflammatory Agents, Non-Steroidal - pharmacology; Antioxidants; Antioxidants - pharmacology; apigenin; Apigenin - pharmacology; Apoptosis; Apoptosis - drug effects; Bax protein; bcl-2-Associated X Protein - agonists; bcl-2-Associated X Protein - antagonists & inhibitors; bcl-2-Associated X Protein - metabolism; Biomarkers - metabolism; Cardiotonic Agents - adverse effects; Cardiotonic Agents - antagonists & inhibitors; Caspase; Cell Line; Cytochrome; Cytochrome c; Cytochromes; Deoxyribonucleic acid; Diet; DNA; DNA damage; DNA Damage - drug effects; fas Receptor - agonists; fas Receptor - antagonists & inhibitors; fas Receptor - metabolism; Gene expression; Inflammation; Inflammation Mediators - agonists; Inflammation Mediators - antagonists & inhibitors; Inflammation Mediators - metabolism; inflammatory markers; Isoproterenol; Isoproterenol - adverse effects; Isoproterenol - antagonists & inhibitors; Lipid Peroxidation - drug effects; Lipids; Membrane Potential, Mitochondrial; Mitochondria, Heart - drug effects; Mitochondria, Heart - immunology; Mitochondria, Heart - metabolism; Mitochondrial Membranes - drug effects; Mitochondrial Membranes - immunology; Mitochondrial Membranes - metabolism; Myoblasts, Cardiac - cytology; Myoblasts, Cardiac - drug effects; Myoblasts, Cardiac - immunology; Myoblasts, Cardiac - metabolism; Oxidative Stress - drug effects; Proteins; Rats; Signaling
• ISSN: 1095-6670; 1099-0461
• DOI: 10.1002/jbt.22213

We investigated the effect of apigenin, a dietary flavonoid, on isoproterenol hydrochloride (ISO)‐induced apoptotic signaling in cardiomyoblast H9C2 cells. The results showed that apigenin treatment (10 µM) prevented ISO (31.25 μM)‐induced lipid peroxidative levels and antioxidants status in H9C2 cells. Furthermore, apigenin inhibited expression of inflammatory markers in ISO‐treated cells. In addition, apigenin prevented ISO‐induced DNA damage and apoptotic signaling through modulating the expression of Bax, caspase‐3, ‐8 and ‐9, cytochrome c, and Fas proteins in H9C2 cells. It is concluded that apigenin prevents ISO‐induced antioxidants depletion, oxidative DNA damage, inflammatory, and apoptotic signaling in H9C2 cells. Thus, the present results demonstrated that apigenin has a cardioprotective effect on cardiomyoblasts cells.