Comprehensive multiphase NMR applied to a living organism

Comprehensive multiphase (CMP) NMR is a novel technology that integrates all the hardware from solution-, gel- and solid-state into a single NMR probe, permitting all phases to be studied in intact samples. Here comprehensive multiphase (CMP) NMR is used to study all components in a living organism...

Full description

Saved in:
Bibliographic Details
Published in:Chemical science (Cambridge) 2016-01, Vol.7 (8), p.4856-4866
Main Authors: Mobarhan, Yalda Liaghati, Fortier-McGill, Blythe, Soong, Ronald, Maas, Werner E, Fey, Michael, Monette, Martine, Stronks, Henry J, Schmidt, Sebastian, Heumann, Hermann, Norwood, Warren, Simpson, André J
Format: Article
Language:English
Subjects:
Binding
Contaminants
In vivo methods and tests
Metabolites
Multiphase
Nuclear magnetic resonance
Organisms
Phases
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Comprehensive multiphase (CMP) NMR is a novel technology that integrates all the hardware from solution-, gel- and solid-state into a single NMR probe, permitting all phases to be studied in intact samples. Here comprehensive multiphase (CMP) NMR is used to study all components in a living organism for the first time. This work describes 4 new scientific accomplishments summarized as: (1) CMP-NMR is applied to a living animal, (2) an effective method to deliver oxygen to the organisms is described which permits longer studies essential for in-depth NMR analysis in general, (3) a range of spectral editing approaches are applied to fully differentiate the various phases solutions (metabolites) through to solids (shell) (4) C isotopic labelling and multidimensional NMR are combined to provide detailed assignment of metabolites and structural components . While not explicitly studied here the multiphase capabilities of the technique offer future possibilities to study kinetic transfer between phases ( nutrient assimilation, contaminant sequestration), molecular binding at interfaces ( drug or contaminant binding) and bonding across and between phases ( muscle to bone) . Future work will need to focus on decreasing the spinning speed to reduce organism stress during analysis.
ISSN:2041-6520
2041-6539
DOI:10.1039/c6sc00329j