Cleavable and thermo-responsive hybrid nanoparticles for on-demand drug delivery

• Published in: Journal of colloid and interface science 2019-01, Vol.533, p.171-181
• Main Authors: Ortiz de Solorzano, Isabel, Alejo, Teresa, Abad, Miriam, Bueno-Alejo, Carlos, Mendoza, Gracia, Andreu, Vanesa, Irusta, Silvia, Sebastian, Victor, Arruebo, Manuel
• Format: Article
• Language: English
• Subjects: Anesthetics, Local - chemistry; Anesthetics, Local - pharmacology; Animals; Apoptosis - drug effects; Bupivacaine; Bupivacaine - chemistry; Bupivacaine - pharmacology; Cell Cycle - drug effects; Cell Survival - drug effects; Cells, Cultured; Cleavable nanoparticles; Copper - chemistry; Drug delivery; Drug Delivery Systems; Humans; Infrared Rays; Mice; Nanoparticles - chemistry; NIR; Particle Size; Photothermal; Sulfides - chemistry; Surface Properties; Temperature; Thermosensitive polymer
• ISSN: 0021-9797; 1095-7103
• DOI: 10.1016/j.jcis.2018.08.069

[Display omitted] By combining the photothermal ability of copper sulphide nanoparticles (NPs) upon excitation with Near Infrared (NIR) Light and the thermo-responsive properties of the homemade oligo (ethylene glycol) methyl ether methacrylate copolymer we have obtained fragmentable nanocomposites able to release a carried drug on-demand after NIR-light triggering. A complete physico-chemical characterization of the resulting nanoparticles has been carried out and their degradation assessed at different temperatures. Herein, we have also evaluated the drug loading capacity of those nanoparticles and the temperature dependence in their drug release kinetics using bupivacaine hydrochloride as a model drug. For those hybrid nanoparticles, subcytotoxic doses on four different cell lines and their potential interference in cell metabolism, induction of apoptosis, and cell cycle have been evaluated by Alamar Blue fluorometry and flow cytometry.