Anti‐Inflammatory Activities and Related Mechanism of Polysaccharides Isolated from Sargentodoxa cuneata

• Published in: Chemistry & biodiversity 2018-11, Vol.15 (11), p.e1800343-n/a
• Main Authors: Guo, Lanfang, Ma, Ruili, Sun, Huifang, Raza, Aun, Tang, Jian, Li, Zhengrong
• Format: Article
• Language: English
• Subjects: Animals; anti-inflammatory activities; Anti-Inflammatory Agents, Non-Steroidal - chemistry; Anti-Inflammatory Agents, Non-Steroidal - isolation & purification; Anti-Inflammatory Agents, Non-Steroidal - pharmacology; Arthritis; Carrageenan; Cell Survival - drug effects; Cells, Cultured; cytotoxicity; Dose-Response Relationship, Drug; Edema; Edema - chemically induced; Edema - drug therapy; Enzyme Inhibitors - chemistry; Enzyme Inhibitors - isolation & purification; Enzyme Inhibitors - pharmacology; Inflammation; iNOS; Lipopolysaccharides; Liver; Macrophages - drug effects; Macrophages - metabolism; Magnoliopsida - chemistry; Male; Malondialdehyde; Mice; Nitric oxide; Nitric Oxide - antagonists & inhibitors; Nitric Oxide - metabolism; Nitric Oxide Synthase Type II - antagonists & inhibitors; Nitric Oxide Synthase Type II - metabolism; Nitric-oxide synthase; polysaccharide; Polysaccharides; Polysaccharides - chemistry; Polysaccharides - isolation & purification; Polysaccharides - pharmacology; Prostaglandin E2; Rats; Rats, Sprague-Dawley; RAW 264.7 Cells; Saccharides; Sargentodoxa cuneata; Structure-Activity Relationship; Superoxide dismutase
• ISSN: 1612-1872; 1612-1880
• DOI: 10.1002/cbdv.201800343

Sargentodoxa cuneata decoction has been used to treat arthritis in China for hundreds of years. Herein, the polysaccharide fraction (PSC) purified from S. cuneata was evaluated for its in vitro and in vivo anti‐inflammatory effects. PSC and its sub‐fractions PSCA‐1 and PSCB‐1 significantly suppressed nitric oxide (NO) release in LPS‐induced RAW264.7 cells by down regulating the inducible nitric oxide synthase (iNOS) level. Furthermore, PSC markedly inhibited carrageenan induced rat hind paw edema, decreased in hind paw, serum and liver malondialdehyde (MDA) levels and prostaglandin E2 (PGE2) levels. In addition, PSC increased superoxide dismutase (SOD) activity in serum and liver of the rats. These results revealed that the polysaccharide obtained from S. cuneata (PSC) possessed potent anti‐inflammatory activity and may be one of the important bioactive constituents from the plant responsible for the anti‐arthritis effect.