Arab-1, a GDSL Lipase from the Model Plant, Arabidopsis thaliana (L.) Heynh.

Bioinformatics analysis of the protein Arab-1 from model plant Arabidopsis thaliana (L.) Heynh. was performed at the level of primary, secondary and tertiary structure. Results suggest that Arab-1 belongs to the important but structurally very poorely characterised GDSL (SGNH) family of hydrolases....

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Bibliographic Details
Published in:Croatica chemica acta 2009-07, Vol.82 (2), p.439-447
Main Authors: Mikleusevic, Goran, Salopek-Sondi, Branka, Luic, Marija
Format: Article
Language:English
Subjects:
Arabidopsis thaliana
Bioinformatics
Brassica napus
Catalysis
Enzyme kinetics
Enzymes
Escherichia coli
Lipids
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Summary:Bioinformatics analysis of the protein Arab-1 from model plant Arabidopsis thaliana (L.) Heynh. was performed at the level of primary, secondary and tertiary structure. Results suggest that Arab-1 belongs to the important but structurally very poorely characterised GDSL (SGNH) family of hydrolases. The amino acids Ser42, Gly118, Asn180, Asp346 and His349 are predicted as the active center residues. According to phylogenetic analysis, Arab-1 is related to Brassica napus lipases. Attempts to produce recombinant Arab-1, using pET and pRSET expression systems, and several strains of Escherichia coli cells are described; the procedures were found to be inadequate for the production of amounts of protein sufficient for crystallisation. The problems observed in protein production are discussed in view of bioinformatics analyses and calculated physico-chemical parameters. [PUBLICATION ABSTRACT]
ISSN:0011-1643
1334-417X