X-ray observations of single bio-supramolecular photochirogenesis

The binding and photochirogenic behaviour of 2-anthracenecarboxylate (AC) with human serum albumin (HSA) have hitherto been investigated and comprehended as time-averaged statistical events by spectroscopic examinations and product analyses. In this study, we employed a diffracted X-ray tracking (DX...

Full description

Saved in:
Bibliographic Details
Published in:Biophysical chemistry 2018-11, Vol.242, p.1-5
Main Authors: Chang, Jaewon, Nishijima, Masaki, Sekiguchi, Hiroshi, Ichiyanagi, Kohei, Kuramochi, Masahiro, Inoue, Yoshihisa, Sasaki, Yuji C.
Format: Article
Language:English
Subjects:
Visualization of high speed photoreaction fast and high resolution measurements single molecule measurements photochirogenesis asymmetric catalyst
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The binding and photochirogenic behaviour of 2-anthracenecarboxylate (AC) with human serum albumin (HSA) have hitherto been investigated and comprehended as time-averaged statistical events by spectroscopic examinations and product analyses. In this study, we employed a diffracted X-ray tracking (DXT) technique to visualize the single-molecular dynamics of free and AC-loaded HSA (AC:HSA = 0, 1, 5 and 10), as well as the AC-HSA complex under photoirradiation, all of which were tethered to gold nanocrystals and hence traceable in real time by DXT. This enabled us to draw a more dynamic picture of the bio-supramolecular photochirogenesis at a single-molecule resolution, detailing the softening and flexibility enhancement of HSA upon binding of ACs to its inter-subdomain IIA-IIB site and the dynamic extrusion of AC dimers produced upon photoirradiation. [Display omitted]
ISSN:0301-4622
1873-4200
DOI:10.1016/j.bpc.2018.07.003