PCR test to specifically detect the apicomplexan 'X' (APX) parasite found in flat oysters Ostrea chilensis in New Zealand

Described here is a polymerase chain reaction (PCR) test to detect the apicomplexan-X (APX) parasite of a flat oyster species, Ostrea chilensis, endemic to New Zealand. The test primers target sequences in the in situ hybridisation probes identified to bind specifically to APX 18S rRNA and amplify a...

Full description

Saved in:
Bibliographic Details
Published in:Diseases of aquatic organisms 2018-08, Vol.129 (3), p.199-205
Main Authors: Suong, Nguyen Thao, Banks, Jonathan C, Webb, Stephen C, Jeffs, Andrew, Wakeman, Kevin C, Fidler, Andrew
Format: Article
Language:English
Subjects:
Amplification
Animals
Apicomplexa - isolation & purification
Base Sequence
Deoxyribonucleic acid
DNA
DNA - genetics
DNA probes
Endemic species
Gene sequencing
Histology
Host-Parasite Interactions
Hybridization
New Zealand
Nucleotide sequence
Ostrea - virology
Ostrea chilensis
Oysters
Polymerase chain reaction
Polymerase Chain Reaction - methods
Primers
rRNA 18S
Sensitivity analysis
Sensitivity and Specificity
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Described here is a polymerase chain reaction (PCR) test to detect the apicomplexan-X (APX) parasite of a flat oyster species, Ostrea chilensis, endemic to New Zealand. The test primers target sequences in the in situ hybridisation probes identified to bind specifically to APX 18S rRNA and amplify a 723 bp DNA product. The test did not amplify 18S rRNA gene sequences of other apicomplexan species, including Toxoplasma gondii, Neospora caninum, Selenidium spp., Cephaloidophorida spp., Lecudina spp. and Thiriotia sp. Of 73 flat oysters identified by histology to be infected with APX at different severities, 69 (95%) tested PCR-positive. Failure to amplify an internal control indicated the presence of PCR inhibitors in the 4 PCR-negative samples. The high analytical sensitivity, specificity and speed of the PCR test should make it a useful tool for detecting APX.
ISSN:0177-5103
1616-1580
DOI:10.3354/dao03244