Effects of estradiol-17 beta and 17 alpha , 20 beta -dihydroxy-4-pregnen-3-one on different phospholipids metabolism and histological changes in ovary during reproductive growth in the catfish, Heteropneustes fossilis (Bloch)

• Published in: Journal of environmental biology 2007-10, Vol.28 (4), p.771-778
• Main Authors: Singh, P B, Singh, V, Srivastava, S, Pandey, S
• Format: Article
• Language: English
• Subjects: Heteropneustes fossilis
• ISSN: 0254-8704

Effect of cumulative doses of estradiol-17 beta (E sub(2) - 7, 14 and 28 mg/kg body weight) and 17 alpha , 20 beta -dihydroxy-4-pregnen-3-one (17 alpha , 20 beta P-7, 14 and 28 mg/kg body weight) on total phospholipids (TP) and various phospholipids- phosphatidylcholine (PC), phosphatidylserine (PS), phosphatidylinositol (PI) and phosphatidylethanolamine(PE) on liver, plasma and ovary were investigated during the reproductively active preparatory and prespawning phases of the annual reproductive cycle in the freshwater female catfish, H. fossilis. The effect of E sub(2) on TP was generally stimulatory and has pronounced effect than 17 alpha , 20 beta P during both the phases. The levels of PC was promoted high during prespawning phase by E sub(2) comparatively very less than by 17 alpha , 20 beta P in studied tissues during both the phases. The levels of PS after E sub(2) treatments was maximum in all tissues during prespawning phase whereas 17 alpha , 20 beta P was effective only in liver during this phase. The PI was elevated in liver during preparatory phase but its elevation was in all studied tissues during prespawning phase after E sub(2) treatments. The levels of PI was most effective in ovary during preparatory phase in response to 17 alpha , 20 beta P. The levels of PE was declined in liver but elevated in ovary after E sub(2) treatments during both the phases. Treatments of E sub(2) during preparatory phase showed greater number of vitellogenic oocytes as compared to 17 alpha , 20 beta P treatments. The present finding has demonstrated that estradiol-17 beta has more pronounced effects than the 17 alpha , 20 beta P in regulation of different phospholipids and ovarian recrudescence during reproductively active phases and among the phospholipids the PC is the main phospholipids of vitellogenin/ovarian lipids in H. fossilis.