Strategies for Creating Structure-Switching Aptamers

Aptamer biosensor that can switch its structure upon target binding offers a powerful strategy for molecular detection. However, the process of converting an aptamer into a “structure-switching” biosensor is challenging and often relies on trial-and-error without established design principles. In th...

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Bibliographic Details
Published in:ACS sensors 2018-09, Vol.3 (9), p.1611-1615
Main Authors: Feagin, Trevor A, Maganzini, Nicolò, Soh, Hyongsok Tom
Format: Article
Language:English
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Summary:Aptamer biosensor that can switch its structure upon target binding offers a powerful strategy for molecular detection. However, the process of converting an aptamer into a “structure-switching” biosensor is challenging and often relies on trial-and-error without established design principles. In this Sensor Issues, we examine a variety of design approaches for incorporating structure-switching functionality into existing aptamers, and provide thermodynamic analyses to highlight the variables that most strongly influence their performance. Finally, we also describe emerging efforts for incorporating the structure-switching functionality directly into the aptamer selection process.
ISSN:2379-3694
2379-3694
DOI:10.1021/acssensors.8b00516