Single Nucleotide Polymorphism in the Cytolethal Distending Toxin B Gene Confers Heterogeneity in the Cytotoxicity of Actinobacillus actinomycetemcomitans

Clinical Actinobacillus actinomycetemcomitans produces cytolethal distending toxin (CDT) with titers ranging from 10² to 10⁸ U/mg. Single nucleotide polymorphism analysis of the cdt gene in clinical isolates identified a variation of a single amino acid at residue 281 of CdtB, which significantly af...

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Bibliographic Details
Published in:Infection and Immunity 2006-12, Vol.74 (12), p.7014-7020
Main Authors: Nishikubo, Shuichi, Ohara, Masaru, Ikura, Masae, Katayanagi, Katsuo, Fujiwara, Tamaki, Komatsuzawa, Hitoshi, Kurihara, Hidemi, Sugai, Motoyuki
Format: Article
Language:English
Subjects:
Actinobacillus actinomycetemcomitans
Aggregatibacter actinomycetemcomitans - genetics
Aggregatibacter actinomycetemcomitans - isolation & purification
Aggregatibacter actinomycetemcomitans - pathogenicity
Amino Acid Sequence
Amino Acid Substitution
Bacterial Toxins - genetics
Bacterial Toxins - toxicity
Bacteriology
Biological and medical sciences
Chromatin - drug effects
Fundamental and applied biological sciences. Psychology
Genes, Bacterial
HeLa Cells
Humans
Microbiology
Miscellaneous
Molecular Pathogenesis
Molecular Sequence Data
Mutagenesis, Site-Directed
Polymorphism, Single Nucleotide
Virulence Factors - genetics
Virulence Factors - toxicity
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Summary:Clinical Actinobacillus actinomycetemcomitans produces cytolethal distending toxin (CDT) with titers ranging from 10² to 10⁸ U/mg. Single nucleotide polymorphism analysis of the cdt gene in clinical isolates identified a variation of a single amino acid at residue 281 of CdtB, which significantly affected CDT toxicity by modulating the chromatin-degrading activity of CdtB.
ISSN:0019-9567
1098-5522
DOI:10.1128/IAI.02063-05