Simultaneous determination of ten neonicotinoid insecticides and two metabolites in honey and Royal-jelly by solid−phase extraction and liquid chromatography−tandem mass spectrometry

•This article describe multiresidue analysis of neonicotinoid insecticides in honey and royal-jelly samples.•This article is the first paper in this field.•A LC–MS/MS method was developed and optimized.•The detailed interpretation of the target analytes MS fragmentation was first provided. An analyt...

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Bibliographic Details
Published in:Food chemistry 2019-01, Vol.270, p.204-213
Main Authors: Hou, Jianbo, Xie, Wen, Hong, Deng, Zhang, Wenhua, Li, Fei, Qian, Yan, Han, Chao
Format: Article
Language:English
Subjects:
Animals
Bees - chemistry
Chromatography, High Pressure Liquid
Chromatography, Liquid
Honey
Honey - analysis
Insecticides - analysis
LC–MS/MS
Limit of Detection
Metabolites
Neonicotinoid insecticides
Neonicotinoids - analysis
Pesticide Residues - analysis
Royal-jelly
Solid Phase Extraction
Tandem Mass Spectrometry
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Summary:•This article describe multiresidue analysis of neonicotinoid insecticides in honey and royal-jelly samples.•This article is the first paper in this field.•A LC–MS/MS method was developed and optimized.•The detailed interpretation of the target analytes MS fragmentation was first provided. An analytical method based on liquid chromatography-tandem mass spectrometry (LC–MS/MS) method was developed for the first simultaneous determination of ten neonicotinoid insecticides (pymetrozine, dinotefuran, nitenpyram, thiamethoxam, flonicamid, imidacloprid, clothianidin, imidaclothiz, acetamiprid, thiacloprid) and two metabolites (4-trifluoromethylnicotinamide and N-desmethylacetamiprid) in honey and royal-jelly. The quantitative detection was performed by LC–MS/MS on multiple reaction monitoring (MRM) mode under positive-ion electrospray ionization. The isotope dilution internal standard method or external standard method was used for quantitation analysis. The limits of quantification (LOQs), calculated as 10 times the standard deviation, were 0.25–5.0 µg kg−1 for ten neonicotinoid insecticides and two metabolites. The average recoveries were in the range of 72.8–106.5%, with relative standard deviations (RSDs) below 13.8%, measured at three concentration levels. The developed method exhibits a high sensitivity and suitable for the simultaneous determination of neonicotinoid insecticides and metabolites in honey and royal-jelly.
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2018.07.068