Cryo-EM structure reveals cylindrical nucleocapsids from two polydnaviruses

Bracovirus is one of the two polydnavirus genera. Here, we used a cryo-EM analysis to reveal the near-native morphology of two nucleocapsid-containing model bracoviruses: Microplitis bicoloratus bracovirus (MbBV) and Microplitis mediator bracovirus (MmBV). MbBV and MmBV nucleocapsids have discernabl...

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Bibliographic Details
Published in:Archives of virology 2018-12, Vol.163 (12), p.3357-3363
Main Authors: Cui, Ji-Hui, Chen, Ya-Bin, Li, Ming, Cai, Qiu-Chen, Zhang, Li-Dan, Lu, Zi-Yun, Li, Jian-Cheng, Zhu, Qi-Shun, Ji, Gang, Luo, Kai-Jun
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Biomedicine
Brief Report
Infectious Diseases
Medical Microbiology
Nucleocapsids
Virology
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Summary:Bracovirus is one of the two polydnavirus genera. Here, we used a cryo-EM analysis to reveal the near-native morphology of two nucleocapsid-containing model bracoviruses: Microplitis bicoloratus bracovirus (MbBV) and Microplitis mediator bracovirus (MmBV). MbBV and MmBV nucleocapsids have discernable cap structures in two distal regions with relatively high electron density. Adjacent to the end-cap structures are two electron-lucent rings. Some nucleocapsids were uniformly electron-dense and had a distinctive “helix-tail-like structure”. Cryo-EM revealed inconsistent nucleocapsid diameters of 34–69.9 nm in MbBV and 46–69.9 nm in MmBV, and the largest observed cylindrical area length was expanded to 126 nm.
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-018-3958-8