Label free DNA-based optical biosensor as a potential system for wine authenticity

•Label-free DNA-based biosensor applied to authenticity.•Varietal identification in different matrices (leaf, must and wine samples)•PCR-free detection. The diversity found among the Vitis vinifera L. species allows the production of wines with very different characteristics. The development of plat...

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Bibliographic Details
Published in:Food chemistry 2019-01, Vol.270, p.299-304
Main Authors: Barrias, Sara, Fernandes, José R., Eiras-Dias, José Eduardo, Brazão, João, Martins-Lopes, Paula
Format: Article
Language:English
Subjects:
Authenticity
Biosensing Techniques
DNA - analysis
DNA-based biosensor
Fruit
Plant Leaves
Vitis - genetics
Vitis vinifera L
Wine
Wine - analysis
Wine - classification
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Summary:•Label-free DNA-based biosensor applied to authenticity.•Varietal identification in different matrices (leaf, must and wine samples)•PCR-free detection. The diversity found among the Vitis vinifera L. species allows the production of wines with very different characteristics. The development of platforms suitable for food composition analysis is currently an emerging area. Among these, DNA biosensors have been developed for a wide variety of applications, ranging from food safety to authenticity. The main aim of this work was to study the detection capacity of the DNA-based optical biosensor using different V. vinifera matrices (leaf, must and wine). Genomic DNA was extracted from leaf, must and wine of three V. vinifera varieties and was tested on the long-period grating (LPG) DNA-based biosensor developed within our group. The biosensor was able to distinguish the varieties even using DNA extracted from complex matrices, revealing its potential to be applied in wine authenticity.
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2018.07.058