Fluor-thiol Photocoupling Reaction for Developing High Performance Nucleic Acid (NA) Microarrays

• Published in: Analytical chemistry (Washington) 2018-10, Vol.90 (19), p.11224-11231
• Main Authors: Jiménez-Meneses, Pilar, Bañuls, María-José, Puchades, Rosa, Maquieira, Ángel
• Format: Article
• Language: English
• Subjects: Anchoring; Biomolecules; Chemical compounds; Chemistry; Deoxyribonucleic acid; DNA; Fabrication; Fluorides; Hybridization; Hydrophobicity; Immobilization; Membranes; Nucleic acids; Polytetrafluoroethylene; Polyvinylidene fluorides; Substrates; Ultraviolet radiation
• ISSN: 0003-2700; 1520-6882
• DOI: 10.1021/acs.analchem.8b00265

Spatially controlled anchoring of NA probes onto microscope glass slides by a novel fluor-thiol coupling reaction is performed. By this UV-initiated reaction, covalent immobilization in very short times (30 s at 254 nm) is achieved with probe densities of up to 39.6 pmol/cm2. Modulating the surface hydrophobicity by combining a hydrophobic silane and a hydrophilic silane allows the fabrication of tuned surfaces where the analyte approaches only the anchored probe, which notably reduces nonspecific adsorption and the background. The generated substrates have proven clear advantages for discriminating single-base-pair mismatches, and for detecting bacterial PCR products. The hybridization sensitivity achieved by these high-performance surfaces is about 1.7 pM. Finally, this anchoring reaction is demonstrated using two additional surfaces: polytetrafluoroethylene (PTFE) and polyvinylidene fluoride (PVDF) membranes. This provides a very interesting pathway for anchoring thiolated biomolecules onto surfaces with C–F motifs via a quick clean UV reaction.