Short-term under-ice variability of prokaryotic plankton communities in coastal Antarctic waters (Cape Hallett, Ross Sea)

• Published in: Estuarine, coastal and shelf science coastal and shelf science, 2009-03, Vol.81 (4), p.491-500
• Main Authors: Celussi, Mauro, Paoli, Alessandro, Crevatin, Erica, Bergamasco, Andrea, Margiotta, Francesca, Saggiomo, Vincenzo, Umani, Serena Fonda, Del Negro, Paola
• Format: Article
• Language: English
• Subjects: Animal and plant ecology; Animal, plant and microbial ecology; Archaea; Bacteria; biogeochemical processes; Biological and medical sciences; Brackish water ecosystems; Flavobacteria; Fundamental and applied biological sciences. Psychology; Marine; PCR–DGGE; Ross Sea; Synecology
• ISSN: 0272-7714; 1096-0015
• DOI: 10.1016/j.ecss.2008.12.014

During the 2006 Italian Antarctic expedition a diel sampling was performed close to Cape Hallett (Ross Sea) during the Austral summer. Under-ice seawater samples (∼4 m) were collected every 2 h for 28 h in order to estimate prokaryotic processes' variability and community structure dynamics. Prokaryotic and viral abundances, exoenzymatic activities (β-glucosidase, chitinase, lipase, alkaline phosphatase and leucine aminopeptidase), prokaryotic carbon production ( 3 H-leucine incorporation) and community structure (Denaturing Gradient Gel Electrophoresis – DGGE fingerprints) were analysed. Results showed that the diel variability of the prokaryotic activity followed a variation in salinity, probably as a consequence of the periodical thawing of sea ice (driven by solar radiation and air temperature cycles), while negligible variation in viral and prokaryotic abundances occurred. The Bacterial and Archaeal community structures underwent an Operational Taxonomic Units (OTUs) temporal shift from the beginning to the end of the sampling, while Flavobacteria-specific primers highlighted high variations in this group possibly related to sea ice melting and substrate release.