MicroRNA-138 regulates cell adhesion-mediated drug resistance phenotype by targeting SGTA in non-Hodgkin's lymphoma

To analyze the effects of miR-138 on the expression of small glutamine-rich TPR-containing protein A (SGTA) and cell adhesion-mediated drug resistance (CAM-DR) phenotype in non-Hodgkin's lymphoma (NHL). The adhesion model was constructed using fibronectin (FN) or bone marrow stromal cells HS-5....

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Bibliographic Details
Published in:Zhōnghuá xuèyèxué zázhì 2018-08, Vol.39 (8), p.668-673
Main Authors: Wu, Y X, He, S, Xu, X H
Format: Article
Language:Chinese
Subjects:
Carrier Proteins
Cell Adhesion
Cell Line, Tumor
Cell Proliferation
Drug Resistance, Neoplasm
Gene Expression Regulation, Neoplastic
Humans
Lymphoma, Non-Hodgkin
MicroRNAs
Phenotype
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Summary:To analyze the effects of miR-138 on the expression of small glutamine-rich TPR-containing protein A (SGTA) and cell adhesion-mediated drug resistance (CAM-DR) phenotype in non-Hodgkin's lymphoma (NHL). The adhesion model was constructed using fibronectin (FN) or bone marrow stromal cells HS-5. The effect of miR-138 on the expression of SGTA was analyzed by Western blotting and RQ-PCR. Dual-luciferase assays were performed to probe the effects of miR-138 on SGTA 3' UTR activities. Subsequently, we investigated the effect of miR-138 on cell cycle, adhesion ability and CAM-DR. Moreover, the correlation between miR-138 expression and therapeutic response was analyzed in 35 paraffin-embedded diffuse large B cell lymphoma samples. Our data showed that adhesion of NHL cells to FN or HS-5 cells significantly increased miR-138 expression (
ISSN:0253-2727
DOI:10.3760/cma.j.issn.0253-2727.2018.08.011