Co-culture of rat luteal cells with islet cells enhances islet viability and revascularization

Islet cell transplantation is a major treatment strategy for type I diabetes, and has proven to be effective for maintaining glucose homeostasis. However, this treatment requires an extended period of immunosuppression to prevent rejection and recurrent transplantation to maintain function. Thus, to...

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Bibliographic Details
Published in:In vitro cellular & developmental biology. Animal 2018-10, Vol.54 (9), p.640-647
Main Authors: Boyuk, Gulbahar, Yigit, A. Arzu, Aydogan, Ilkay
Format: Article
Language:English
Subjects:
Angiogenesis
Animal Genetics and Genomics
Animals
Biomedical and Life Sciences
CELL AND TISSUE MODELS
Cell Biology
Cell Culture
Coculture Techniques
Corpus luteum
Developmental Biology
Diabetes
Diabetes mellitus
Female
Fibroblast growth factor 2
Fibroblast Growth Factor 2 - metabolism
Fibroblasts
Gene expression
Glucose
Graft rejection
Growth factors
Homeostasis
Hypoxia
Immunosuppression
Insulin
Insulin - metabolism
Islet cells
Islets of Langerhans - blood supply
Life Sciences
Luteal Cells - cytology
Mice
Neovascularization, Physiologic
Pancreatic islet transplantation
Pancreatitis
Platelet Endothelial Cell Adhesion Molecule-1 - metabolism
Progesterone
Progesterone - metabolism
Propidium - metabolism
Rats, Sprague-Dawley
Rodents
Stem Cells
Success
Tissue Survival
Transplantation
Transplants & implants
Vascular endothelial growth factor
Vascular Endothelial Growth Factor A - metabolism
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Summary:Islet cell transplantation is a major treatment strategy for type I diabetes, and has proven to be effective for maintaining glucose homeostasis. However, this treatment requires an extended period of immunosuppression to prevent rejection and recurrent transplantation to maintain function. Thus, to enhance the properties of transplanted islet cells, we examined the effect of the co-culture of luteal cells, which secrete progesterone, on islet cell viability, functionality, and revascularization. It was found that islet viability and functionality were higher in the co-cultured group than in single cultures of islets at 48 and 96 h, in parallel with increased progesterone and vascular endothelial growth factor (VEGF) secretion from luteal cells. In the co-culture groups, VEGF levels at 48 and 96 h and CD31 levels at 48 h were significantly higher than those in the islet groups (p
ISSN:1071-2690
1543-706X
DOI:10.1007/s11626-018-0286-y