Proliferation in the vomeronasal organ of the rat during postnatal development

• Published in: The European journal of neuroscience 1999-02, Vol.11 (2), p.700-711
• Main Authors: Weiler, Elke, McCulloch, Mary A., Farbman, Albert I.
• Format: Article
• Language: English
• Subjects: ageing; Aging - physiology; Animals; Antimetabolites; Bromodeoxyuridine; Cell Count; Cell cycle; Cell Division - physiology; cell turnover; Epithelial Cells - chemistry; Epithelial Cells - cytology; Female; Jacobson's organ; Male; neurogenesis; Neurons, Afferent - chemistry; Neurons, Afferent - cytology; Rats; Rats, Sprague-Dawley; Sex Factors; Sexes; sexual differences; Smell - physiology; Vomeronasal Organ - cytology; Vomeronasal Organ - growth & development; Weaning
• ISSN: 0953-816X; 1460-9568
• DOI: 10.1046/j.1460-9568.1999.00476.x

We investigated proliferation of sensory cell precursors in the rat vomeronasal organ (VNO) at various postnatal ages from birth (P1) to P666. In the rat, which continues to grow during most of its adult life, proliferation might be related to growth and/or replacement. Proliferating cells were labelled by BrdU injection, and histological sections of the VNO were evaluated after immunohistochemical detection of BrdU. Proliferation density (number of proliferating cells/section) decreased dramatically from 115 at P1 to 27.2 at P21, although the area increased. Adult values were reached at P66–P333 (10.3 cells/section); at P400–P666 the value was 8.6 cells/section. Distribution of labelled cells changed considerably with age: in neonates the cells were nearly equally distributed throughout the sensory epithelium, whereas from P21 onwards most proliferating cells were concentrated in clusters near the boundaries with non‐sensory epithelium. Labelled cells in the sensory neuronal layer were adjacent to the undulating basement membrane‐bordering capillaries that intrude into the sensory epithelium, indicating that they were true basal cells. The volume of the sensory epithelium increased between P1 and P66, and remained constant thereafter, although the length still increased. Length and volume of the sensory epithelium were related to body size, not to sex; males and females of the same body size had the same VNO size. The complex changes in proliferation pattern during postnatal development indicate differential growth and replacement. We suggest that in adults the labelled cell clusters near the boundaries are a pool for growth, whereas proliferation in the central parts represents a replacement pool.