Aberrant Stamen Development is Associated with Parthenocarpic Fruit Set Through Up-Regulation of Gibberellin Biosynthesis in Tomato

• Published in: Plant and cell physiology 2019-01, Vol.60 (1), p.38-51
• Main Authors: Okabe, Yoshihiro, Yamaoka, Tatsuya, Ariizumi, Tohru, Ushijima, Koichiro, Kojima, Mikiko, Takebayashi, Yumiko, Sakakibara, Hitoshi, Kusano, Miyako, Shinozaki, Yoshihito, Pulungan, Sri Imriani, Kubo, Yasutaka, Nakano, Ryohei, Ezura, Hiroshi
• Format: Article
• Language: English
• Subjects: Base Sequence; Biosynthetic Pathways - genetics; Flowers - genetics; Flowers - growth & development; Fruit - genetics; Fruit - growth & development; Gene Expression Regulation, Plant; Gibberellins - biosynthesis; Lycopersicon esculentum - genetics; Lycopersicon esculentum - growth & development; Mutagenesis - genetics; Mutation - genetics; Organ Specificity - genetics; Parthenogenesis - genetics; Phenotype; Plant Growth Regulators - metabolism; Plant Proteins - genetics; Plant Proteins - metabolism; Plants, Genetically Modified; Promoter Regions, Genetic - genetics; RNA Interference; Signal Transduction; Transcription, Genetic; Up-Regulation - genetics
• ISSN: 0032-0781; 1471-9053
• DOI: 10.1093/pcp/pcy184

Abstract Parthenocarpy, a process in which fruit set occurs without fertilization, leads to the production of seedless fruit. A number of floral homeotic mutants with abnormal stamen development exhibit parthenocarpic fruit set. Flower development is thought to repress ovary growth before anthesis. However, the mechanism of parthenocarpic fruit development caused by aberrant flower formation is poorly understood. To investigate the molecular mechanism of parthenocarpic fruit development in floral homeotic mutants, we performed functional analysis of Tomato APETALA3 (TAP3) by loss-of-function approaches. Organ-specific promoter was used to induce organ-specific loss of function in stamen and ovary/fruit. We observed increased cell expansion in tap3 mutants and TAP3-RNAi lines during parthenocarpic fruit growth. These were predominantly accompanied by the up-regulation of GA biosynthesis genes, including SlGA20ox1, SlGA20ox2, and SlGA20ox3, as well as reduced expression of the GA-inactivating gene SlGA2ox1 and the auxin signaling gene SlARF7 involved in a crosstalk between GA and auxin. These transcriptional profiles are in agreement with the GA levels in these lines. These results suggest that stamen development negatively regulates fruit set by repressing the GA biosynthesis.