High-Throughput Sequencing Analysis of the Bacterial Community in Stone Fruit Phloem Tissues Infected by “Candidatus Phytoplasma prunorum”

“Candidatus Phytoplasma prunorum” (CPp) is a highly destructive phytopathogenic agent in many stone fruit-growing regions in Europe and the surrounding countries. In this work, we focused on documenting entire bacterial community in the phloem tissues of 60 stone fruit trees. Nested PCR and two real...

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Bibliographic Details
Published in:Microbial ecology 2019-04, Vol.77 (3), p.664-675
Main Authors: Eichmeier, Ales, Kiss, Tomas, Necas, Tomas, Penazova, Eliska, Tekielska, Dorota, Bohunicka, Marketa, Valentova, Lucie, Cmejla, Radek, Morais, Daniel, Baldrian, Petr
Format: Article
Language:English
Subjects:
Bacteria
Biomedical and Life Sciences
Candidatus Phytoplasma prunorum
Communities
Composition
DNA
Ecology
Fruit trees
Fruits
Geoecology/Natural Processes
Life Sciences
Microbial Ecology
Microbiology
Nature Conservation
Next-generation sequencing
Nucleotide sequence
PCR
Phloem
Phytoplankton
Phytoplasma
PLANT MICROBE INTERACTIONS
Sequencing
Statistical analysis
Statistical methods
Stone
Tissue
Tissues
Trees
Water Quality/Water Pollution
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Summary:“Candidatus Phytoplasma prunorum” (CPp) is a highly destructive phytopathogenic agent in many stone fruit-growing regions in Europe and the surrounding countries. In this work, we focused on documenting entire bacterial community in the phloem tissues of 60 stone fruit trees. Nested PCR and two real-time PCR assays were used to select CPp-positive (group A) and CPp-negative samples (group B). Afterwards, high-throughput amplicon sequencing was performed to assess bacterial community compositions in phloem tissues. The bacterial composition in phloem tissue consisted of 118 distinct genera, represented mainly by Pseudomonas, Acinetobacter, Methylobacterium, Sphingomonas, and Rhizobium. Statistics showed that CPp influenced the bacterial composition of infected plants (group A) and that the bacterial community depended on the geographical origin of the sample. This is the first work focusing on an analysis of the influence of CPp on the bacteria coexisting in the phloem tissues of stone fruit trees.
ISSN:0095-3628
1432-184X
DOI:10.1007/s00248-018-1250-9