Antioxidant Activity of Hydrogen Nanobubbles in Water with Different Reactive Oxygen Species both in Vivo and in Vitro

Hydrogen water as a new therapeutic antioxidant has been widely used in living organisms under stress. In this study, we applied nanobubble (NB) technology to hydrogen water. The antioxidant capacity of hydrogen NB water was studied with respect to different reactive oxygen species (ROS) both in vit...

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Bibliographic Details
Published in:Langmuir 2018-10, Vol.34 (39), p.11878-11885
Main Authors: Liu, Shu, Oshita, Seiichi, Thuyet, Dang Quoc, Saito, Masanao, Yoshimoto, Takahiko
Format: Article
Language:English
Subjects:
Aniline Compounds - chemistry
Fluoresceins - chemistry
Fluorescent Dyes - chemistry
Free Radical Scavengers - chemistry
Free Radical Scavengers - pharmacology
Germination - drug effects
Hordeum - metabolism
Hydrogen - chemistry
Hydrogen - pharmacology
Hydrogen Peroxide - chemistry
Hydrogen Peroxide - metabolism
Hydroxyl Radical - chemistry
Hypochlorous Acid - chemistry
Nanostructures - chemistry
Peroxynitrous Acid - chemistry
Seeds - metabolism
Superoxides - chemistry
Superoxides - metabolism
Water - chemistry
Water - pharmacology
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Summary:Hydrogen water as a new therapeutic antioxidant has been widely used in living organisms under stress. In this study, we applied nanobubble (NB) technology to hydrogen water. The antioxidant capacity of hydrogen NB water was studied with respect to different reactive oxygen species (ROS) both in vitro and in vivo. Using a relatively weak reduced dye, APF, we showed that hydrogen NB water can effectively remove three cytotoxic ROS, •OH, ClO–, and ONOO–, from water. Hydrogen NB water could also remove O2 •–, which is a physiologically important ROS, from water. However, hydrogen water could not reduce other physiologically important ROS such as H2O2 and NO. At similar dissolved hydrogen concentrations, hydrogen NB water displayed higher antioxidant activity than hydrogen water without NB. Barley seed germination tests were used to study the antioxidant effect of hydrogen NB water on ROS generation in vivo. Our results showed that this decreased the physiological activity of barley seeds in their normal homeostatic state. Hydrogen NB water eliminated endogenous O2 •– in seeds and inhibited germination. The usage of hydrogen NB water should be individually considered according to the types of cells involved. Our results offer basic data concerning the application of hydrogen NB water in different fields.
ISSN:0743-7463
1520-5827
DOI:10.1021/acs.langmuir.8b02440