IL-2-induced NF-κB phosphorylation upregulates cation nonselective conductance in human cardiac fibroblasts

• Published in: International immunopharmacology 2018-11, Vol.64, p.170-174
• Main Authors: Mitrokhin, Vadim, Gorbacheva, Lyubov, Mladenov, Mitko, Kamkin, Andre
• Format: Article
• Language: English
• Subjects: Calcium - metabolism; Cardiac arrhythmia; Cation nonselective conductance; Cations - metabolism; Cell culture; Cells, Cultured; Conductance; Depolarization; Electric Conductivity; Endoplasmic Reticulum - physiology; Enzyme-linked immunosorbent assay; Fibroblasts; Fibroblasts - metabolism; Heart; Human cardiac fibroblasts; Humans; IL-2; Immunotherapy; Interleukin 2; Interleukin-2 - pharmacology; Myocardium - metabolism; NF-kappa B - metabolism; NF-κB and IκB; NF-κB protein; Perfusion; Pharmacology; Phosphorylation; PKC; Prevention; Protein kinase C; Protein Kinase C - physiology; Resistance; Up-Regulation
• ISSN: 1567-5769; 1878-1705
• DOI: 10.1016/j.intimp.2018.08.040

Studies of negative ionotropic effects of IL-2 create the basis for possible IL-2 impact on nonselective conductance (GNS), which potentially makes these effects useful in elucidation of the pathways affected by IL-2. A culture of human cardiac fibroblasts (CHCFs) was used in this study. A voltage clamp mode of the whole-cell patch-clamp technique was introduced. The level of phosphorylated NF-κB was determined by newly developed semi-quantitative ELISA. The IL-2 (5 ng/ml) increased the currents during the depolarizing clamp to larger amplitudes without changing their time course. In the CHCFs pretreated with 50 μmol/L 2-APB, IL-2-induced increase in GNS was highly prevented (p