The control of root-knot nematodes (Meloidogyne spp.) by Pseudomonas oryzihabitans and its immunological detection on tomato roots

Abstract Pseudomonas oryzihabitans, a bacterium associated with the entomopathogenic nematode Steinernema abbasi, was evaluated for its potential to colonise roots and thereby control a field population of root-knot nematodes. Immunological techniques were developed to detect root colonisation of P....

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Bibliographic Details
Published in:Nematology : international journal of fundamental and applied nematological research 2007-01, Vol.9 (3), p.363-370
Main Authors: Vagelas, Ioannis K, Pembroke, Barbara, Gowen, Simon R, Davies, Keith G
Format: Article
Language:English
Subjects:
Biological and medical sciences
BIOLOGICAL CONTROL
Control
ELISA
FLUORESCENCE MICROSCOPY
Fundamental and applied biological sciences. Psychology
IMMUNO-BLOTTING
Lycopersicon esculentum
Meloidogyne
Nematoda
Phytopathology. Animal pests. Plant and forest protection
Protozoa. Invertebrates
Pseudomonas
RHIZOSPHERE
Steinernema
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Summary:Abstract Pseudomonas oryzihabitans, a bacterium associated with the entomopathogenic nematode Steinernema abbasi, was evaluated for its potential to colonise roots and thereby control a field population of root-knot nematodes. Immunological techniques were developed to detect root colonisation of P. oryzihabitans on tomato roots using a specific polyclonal antibody raised against vegetative bacterial cells. In vitro, bacterial cell filtrates were also shown significantly to inhibit juveniles hatching. In a glasshouse pot experiment, there were 22 and 82% fewer females in roots of plants treated with suspensions containing 103 and 106 cells ml−1 of P. oryzihabitans, respectively. In addition, there were significantly fewer egg masses produced; however, the numbers of eggs per egg mass did not differ significantly. The relationship between root colonisation and nematode control is discussed.
ISSN:1388-5545
1568-5411
DOI:10.1163/156854107781352061