Photobiomodulation therapy improves multilineage differentiation of dental pulp stem cells in three-dimensional culture model

Photobiomodulation therapy (PBM) has shown positive effects on stem cell differentiation in monolayer cell culture model, but little is known about its effect on three-dimensional (3-D) agarose gel culture. This study evaluated the PBM effect of human dental pulp stem cells (hDPSCs) differentiation...

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Bibliographic Details
Published in:Journal of biomedical optics 2018-09, Vol.23 (9), p.095001-095001
Main Authors: Zaccara, Ivana Maria, Mestieri, Letícia Boldrin, Moreira, Maria Stella, Grecca, Fabiana Soares, Martins, Manoela Domingues, Kopper, Patrícia Maria Poli
Format: Article
Language:English
Subjects:
Adolescent
Cell Culture Techniques - methods
Cell Differentiation - radiation effects
Cells, Cultured
Dental Pulp - cytology
Humans
Low-Level Light Therapy
Osteoblasts - radiation effects
Osteogenesis - radiation effects
Stem Cells - radiation effects
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Summary:Photobiomodulation therapy (PBM) has shown positive effects on stem cell differentiation in monolayer cell culture model, but little is known about its effect on three-dimensional (3-D) agarose gel culture. This study evaluated the PBM effect of human dental pulp stem cells (hDPSCs) differentiation and phosphatase alkaline activity (ALP) using an agarose 3-D model under different nutritional conditions. hDPSCs were characterized and seeded on a 0.3% agarose gel layer with different media (osteogenic, adipogenic, chondrogenic) and were assigned into four groups: control 10% fetal bovine serum (FBS), control 5% FBS, PBM 10% FBS, and PBM 5% FBS. Irradiation was performed with continuous-wave InGaAlP laser, 660 nm, 100 mW, 3,3  J  /  cm2, spot size 0.3  cm2, 10 s of exposure time, and 1 J of energy per point with 6-h interval between sessions. All groups were evaluated at 7 and 14 days. ALP assay was performed to analyze the deposition of mineralized tissue. At 7 days, PBM 5% FBS group presented better stimulation in osteogenic and adipogenic differentiation compared with control. After 14 days, hDPSCs cultured in 3-D exhibited osteogenic, adipogenic, and chondrogenic differentiation; furthermore, compared to control, PBM significantly stimulated all differentiation processes (p  
ISSN:1083-3668
1560-2281
DOI:10.1117/1.JBO.23.9.095001