Rapid diagnosis of Zika virus through saliva and urine by Loop-mediated isothermal amplification (LAMP)

Background: Zika virus (ZIKV) is a single-stranded RNA virus and member of the Flaviviridae family. Recent studies have reported that saliva can be an important alternative to detect ZIKV. Saliva requires less processing than blood greatly simplifying the assay. Loop-mediated Isothermal Amplificatio...

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Bibliographic Details
Published in:Journal of oral microbiology 2018-01, Vol.10 (1), p.1510712-6
Main Authors: Castro, Talita, Sabalza, Maite, Barber, Cheryl, Abrams, William, Da Costa, Antonio Charlys, De Pádua Milagres, Flavio Augusto, Braz-Silva, Paulo Henrique, Malamud, Daniel, Gallottini, Marina
Format: Article
Language:English
Subjects:
Blood
Dengue
Dentistry
Flavivirus
Guillain-Barre syndrome
infection
Infections
LAMP
Nucleic acids
Original
Polymerase chain reaction
Ribonucleic acid
RNA
RNA viruses
RNA-directed DNA polymerase
Saliva
Studies
Thermal cycling
Urine
Vector-borne diseases
virology
Viruses
Zika
Zika virus
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Summary:Background: Zika virus (ZIKV) is a single-stranded RNA virus and member of the Flaviviridae family. Recent studies have reported that saliva can be an important alternative to detect ZIKV. Saliva requires less processing than blood greatly simplifying the assay. Loop-mediated Isothermal Amplification (LAMP) is a rapid assay that detects nucleic acids, including ZIKV RNA. Aim: The aim of this study was to evaluate the efficacy of saliva and urine to diagnose ZIKV infection in subjects during the acute phase, through ZIKV RNA detection by LAMP. Method: A total of 131 samples (68 saliva and 63 urine samples) from 69 subjects in the acute phase of ZIKV infection, and confirmed positive for ZIKV by blood analysis through real time-PCR, were collected and analyzed by Reverse Transcriptase Loop-mediated Isothermal Amplification (RT-LAMP). Results: From the 68 saliva samples, 45 (66.2%) were positive for ZIKV with an average time to positivity (Tp) of 13.5 min, and from the 63 urine samples, 25 (39.7%) were positive with the average Tp of 15.8 min. Saliva detected more samples (p = 0.0042) and had faster Tp (p = 0.0176) as compared with urine. Conclusion: Saliva proved to be a feasible alternative to diagnose ZIKV infection during the acute phase by LAMP.
ISSN:2000-2297
2000-2297
DOI:10.1080/20002297.2018.1510712