Rapid diagnosis of Zika virus through saliva and urine by Loop-mediated isothermal amplification (LAMP)

Background: Zika virus (ZIKV) is a single-stranded RNA virus and member of the Flaviviridae family. Recent studies have reported that saliva can be an important alternative to detect ZIKV. Saliva requires less processing than blood greatly simplifying the assay. Loop-mediated Isothermal Amplificatio...

Full description

Saved in:
Bibliographic Details
Published in:Journal of oral microbiology 2018-01, Vol.10 (1), p.1510712-6
Main Authors: Castro, Talita, Sabalza, Maite, Barber, Cheryl, Abrams, William, Da Costa, Antonio Charlys, De Pádua Milagres, Flavio Augusto, Braz-Silva, Paulo Henrique, Malamud, Daniel, Gallottini, Marina
Format: Article
Language:English
Subjects:
Blood
Dengue
Dentistry
Flavivirus
Guillain-Barre syndrome
infection
Infections
LAMP
Nucleic acids
Original
Polymerase chain reaction
Ribonucleic acid
RNA
RNA viruses
RNA-directed DNA polymerase
Saliva
Studies
Thermal cycling
Urine
Vector-borne diseases
virology
Viruses
Zika
Zika virus
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c590t-4ca28dc3eb24354b9e7a10a7c43e5f2907c1481590bfa83779b6426d67410c7a3
cites cdi_FETCH-LOGICAL-c590t-4ca28dc3eb24354b9e7a10a7c43e5f2907c1481590bfa83779b6426d67410c7a3
container_end_page 6
container_issue 1
container_start_page 1510712
container_title Journal of oral microbiology
container_volume 10
creator Castro, Talita
Sabalza, Maite
Barber, Cheryl
Abrams, William
Da Costa, Antonio Charlys
De Pádua Milagres, Flavio Augusto
Braz-Silva, Paulo Henrique
Malamud, Daniel
Gallottini, Marina
description Background: Zika virus (ZIKV) is a single-stranded RNA virus and member of the Flaviviridae family. Recent studies have reported that saliva can be an important alternative to detect ZIKV. Saliva requires less processing than blood greatly simplifying the assay. Loop-mediated Isothermal Amplification (LAMP) is a rapid assay that detects nucleic acids, including ZIKV RNA. Aim: The aim of this study was to evaluate the efficacy of saliva and urine to diagnose ZIKV infection in subjects during the acute phase, through ZIKV RNA detection by LAMP. Method: A total of 131 samples (68 saliva and 63 urine samples) from 69 subjects in the acute phase of ZIKV infection, and confirmed positive for ZIKV by blood analysis through real time-PCR, were collected and analyzed by Reverse Transcriptase Loop-mediated Isothermal Amplification (RT-LAMP). Results: From the 68 saliva samples, 45 (66.2%) were positive for ZIKV with an average time to positivity (Tp) of 13.5 min, and from the 63 urine samples, 25 (39.7%) were positive with the average Tp of 15.8 min. Saliva detected more samples (p = 0.0042) and had faster Tp (p = 0.0176) as compared with urine. Conclusion: Saliva proved to be a feasible alternative to diagnose ZIKV infection during the acute phase by LAMP.
doi_str_mv 10.1080/20002297.2018.1510712
format article
fullrecord <record><control><sourceid>proquest_infor</sourceid><recordid>TN_cdi_proquest_miscellaneous_2102327942</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_572eb1c1b3154d55b9e317c75a50089d</doaj_id><sourcerecordid>2102327942</sourcerecordid><originalsourceid>FETCH-LOGICAL-c590t-4ca28dc3eb24354b9e7a10a7c43e5f2907c1481590bfa83779b6426d67410c7a3</originalsourceid><addsrcrecordid>eNqFkk1v1DAQhiMEolXpTwBZ4lIOWfwZxxdEVfFRaREIwYWLNXGcrJfE3trJov33eNlt1XKAk-3xM-_M2G9RPCd4QXCNX1OMMaVKLigm9YIIgiWhj4rTfbzcXzy-tz8pzlNa5xNmtK45fVqcMEwxFbg6LfqvsHEtah30PiSXUOjQD_cT0NbFOaFpFcPcr1CCwW0BgW_RHJ23qNmhZQibcrQ5dbItcilMKxtHGBCMm8F1zsDkgkcXy8tPX149K550MCR7flzPiu_v3327-lguP3-4vrpclkYoPJXcAK1bw2xDORO8UVYCwSANZ1Z0VGFpCK9JZpsOaialaipOq7aSnGAjgZ0V1wfdNsBab6IbIe50AKf_BELsNcTJmcFqIaltiCENI4K3QuRijEgjBQiMa9VmrTcHrc3c5DmN9VOE4YHowxvvVroPW10RKnNzWeDiKBDDzWzTpEeXjB0G8DbMSVOCKaNScZrRl3-h6zBHn58qU0owJZn6DyUJoYRxlSlxoEwMKUXb3bVMsN77R9_6R-_9o4_-yXkv7s97l3Xrlgy8PQDOdyF_9a8Qh1ZPsBtC7CJ445Jm_67xG0H20cY</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2171121349</pqid></control><display><type>article</type><title>Rapid diagnosis of Zika virus through saliva and urine by Loop-mediated isothermal amplification (LAMP)</title><source>Publicly Available Content Database</source><source>PubMed Central(OpenAccess)</source><source>Taylor &amp; Francis Open Access(OpenAccess)</source><creator>Castro, Talita ; Sabalza, Maite ; Barber, Cheryl ; Abrams, William ; Da Costa, Antonio Charlys ; De Pádua Milagres, Flavio Augusto ; Braz-Silva, Paulo Henrique ; Malamud, Daniel ; Gallottini, Marina</creator><creatorcontrib>Castro, Talita ; Sabalza, Maite ; Barber, Cheryl ; Abrams, William ; Da Costa, Antonio Charlys ; De Pádua Milagres, Flavio Augusto ; Braz-Silva, Paulo Henrique ; Malamud, Daniel ; Gallottini, Marina</creatorcontrib><description>Background: Zika virus (ZIKV) is a single-stranded RNA virus and member of the Flaviviridae family. Recent studies have reported that saliva can be an important alternative to detect ZIKV. Saliva requires less processing than blood greatly simplifying the assay. Loop-mediated Isothermal Amplification (LAMP) is a rapid assay that detects nucleic acids, including ZIKV RNA. Aim: The aim of this study was to evaluate the efficacy of saliva and urine to diagnose ZIKV infection in subjects during the acute phase, through ZIKV RNA detection by LAMP. Method: A total of 131 samples (68 saliva and 63 urine samples) from 69 subjects in the acute phase of ZIKV infection, and confirmed positive for ZIKV by blood analysis through real time-PCR, were collected and analyzed by Reverse Transcriptase Loop-mediated Isothermal Amplification (RT-LAMP). Results: From the 68 saliva samples, 45 (66.2%) were positive for ZIKV with an average time to positivity (Tp) of 13.5 min, and from the 63 urine samples, 25 (39.7%) were positive with the average Tp of 15.8 min. Saliva detected more samples (p = 0.0042) and had faster Tp (p = 0.0176) as compared with urine. Conclusion: Saliva proved to be a feasible alternative to diagnose ZIKV infection during the acute phase by LAMP.</description><identifier>ISSN: 2000-2297</identifier><identifier>EISSN: 2000-2297</identifier><identifier>DOI: 10.1080/20002297.2018.1510712</identifier><identifier>PMID: 30202506</identifier><language>eng</language><publisher>United States: Taylor &amp; Francis</publisher><subject>Blood ; Dengue ; Dentistry ; Flavivirus ; Guillain-Barre syndrome ; infection ; Infections ; LAMP ; Nucleic acids ; Original ; Polymerase chain reaction ; Ribonucleic acid ; RNA ; RNA viruses ; RNA-directed DNA polymerase ; Saliva ; Studies ; Thermal cycling ; Urine ; Vector-borne diseases ; virology ; Viruses ; Zika ; Zika virus</subject><ispartof>Journal of oral microbiology, 2018-01, Vol.10 (1), p.1510712-6</ispartof><rights>2018 The Author(s). Published by Informa UK Limited, trading as Taylor &amp; Francis Group. 2018</rights><rights>Copyright Taylor &amp; Francis Ltd. 2018</rights><rights>2018 The Author(s). Published by Informa UK Limited, trading as Taylor &amp; Francis Group. This work is licensed under the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2018 The Author(s). Published by Informa UK Limited, trading as Taylor &amp; Francis Group. 2018 The Author(s)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c590t-4ca28dc3eb24354b9e7a10a7c43e5f2907c1481590bfa83779b6426d67410c7a3</citedby><cites>FETCH-LOGICAL-c590t-4ca28dc3eb24354b9e7a10a7c43e5f2907c1481590bfa83779b6426d67410c7a3</cites><orcidid>0000-0001-5516-5177 ; 0000-0003-4019-376X ; 0000-0002-1842-9521 ; 0000-0002-2159-507X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2171121349/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2171121349?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27502,27924,27925,37012,37013,44590,53791,53793,59143,59144,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30202506$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Castro, Talita</creatorcontrib><creatorcontrib>Sabalza, Maite</creatorcontrib><creatorcontrib>Barber, Cheryl</creatorcontrib><creatorcontrib>Abrams, William</creatorcontrib><creatorcontrib>Da Costa, Antonio Charlys</creatorcontrib><creatorcontrib>De Pádua Milagres, Flavio Augusto</creatorcontrib><creatorcontrib>Braz-Silva, Paulo Henrique</creatorcontrib><creatorcontrib>Malamud, Daniel</creatorcontrib><creatorcontrib>Gallottini, Marina</creatorcontrib><title>Rapid diagnosis of Zika virus through saliva and urine by Loop-mediated isothermal amplification (LAMP)</title><title>Journal of oral microbiology</title><addtitle>J Oral Microbiol</addtitle><description>Background: Zika virus (ZIKV) is a single-stranded RNA virus and member of the Flaviviridae family. Recent studies have reported that saliva can be an important alternative to detect ZIKV. Saliva requires less processing than blood greatly simplifying the assay. Loop-mediated Isothermal Amplification (LAMP) is a rapid assay that detects nucleic acids, including ZIKV RNA. Aim: The aim of this study was to evaluate the efficacy of saliva and urine to diagnose ZIKV infection in subjects during the acute phase, through ZIKV RNA detection by LAMP. Method: A total of 131 samples (68 saliva and 63 urine samples) from 69 subjects in the acute phase of ZIKV infection, and confirmed positive for ZIKV by blood analysis through real time-PCR, were collected and analyzed by Reverse Transcriptase Loop-mediated Isothermal Amplification (RT-LAMP). Results: From the 68 saliva samples, 45 (66.2%) were positive for ZIKV with an average time to positivity (Tp) of 13.5 min, and from the 63 urine samples, 25 (39.7%) were positive with the average Tp of 15.8 min. Saliva detected more samples (p = 0.0042) and had faster Tp (p = 0.0176) as compared with urine. Conclusion: Saliva proved to be a feasible alternative to diagnose ZIKV infection during the acute phase by LAMP.</description><subject>Blood</subject><subject>Dengue</subject><subject>Dentistry</subject><subject>Flavivirus</subject><subject>Guillain-Barre syndrome</subject><subject>infection</subject><subject>Infections</subject><subject>LAMP</subject><subject>Nucleic acids</subject><subject>Original</subject><subject>Polymerase chain reaction</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA viruses</subject><subject>RNA-directed DNA polymerase</subject><subject>Saliva</subject><subject>Studies</subject><subject>Thermal cycling</subject><subject>Urine</subject><subject>Vector-borne diseases</subject><subject>virology</subject><subject>Viruses</subject><subject>Zika</subject><subject>Zika virus</subject><issn>2000-2297</issn><issn>2000-2297</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>0YH</sourceid><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNqFkk1v1DAQhiMEolXpTwBZ4lIOWfwZxxdEVfFRaREIwYWLNXGcrJfE3trJov33eNlt1XKAk-3xM-_M2G9RPCd4QXCNX1OMMaVKLigm9YIIgiWhj4rTfbzcXzy-tz8pzlNa5xNmtK45fVqcMEwxFbg6LfqvsHEtah30PiSXUOjQD_cT0NbFOaFpFcPcr1CCwW0BgW_RHJ23qNmhZQibcrQ5dbItcilMKxtHGBCMm8F1zsDkgkcXy8tPX149K550MCR7flzPiu_v3327-lguP3-4vrpclkYoPJXcAK1bw2xDORO8UVYCwSANZ1Z0VGFpCK9JZpsOaialaipOq7aSnGAjgZ0V1wfdNsBab6IbIe50AKf_BELsNcTJmcFqIaltiCENI4K3QuRijEgjBQiMa9VmrTcHrc3c5DmN9VOE4YHowxvvVroPW10RKnNzWeDiKBDDzWzTpEeXjB0G8DbMSVOCKaNScZrRl3-h6zBHn58qU0owJZn6DyUJoYRxlSlxoEwMKUXb3bVMsN77R9_6R-_9o4_-yXkv7s97l3Xrlgy8PQDOdyF_9a8Qh1ZPsBtC7CJ445Jm_67xG0H20cY</recordid><startdate>20180101</startdate><enddate>20180101</enddate><creator>Castro, Talita</creator><creator>Sabalza, Maite</creator><creator>Barber, Cheryl</creator><creator>Abrams, William</creator><creator>Da Costa, Antonio Charlys</creator><creator>De Pádua Milagres, Flavio Augusto</creator><creator>Braz-Silva, Paulo Henrique</creator><creator>Malamud, Daniel</creator><creator>Gallottini, Marina</creator><general>Taylor &amp; Francis</general><general>Taylor &amp; Francis Ltd</general><general>Taylor &amp; Francis Group</general><scope>0YH</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7T7</scope><scope>7X7</scope><scope>7XB</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M7P</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0001-5516-5177</orcidid><orcidid>https://orcid.org/0000-0003-4019-376X</orcidid><orcidid>https://orcid.org/0000-0002-1842-9521</orcidid><orcidid>https://orcid.org/0000-0002-2159-507X</orcidid></search><sort><creationdate>20180101</creationdate><title>Rapid diagnosis of Zika virus through saliva and urine by Loop-mediated isothermal amplification (LAMP)</title><author>Castro, Talita ; Sabalza, Maite ; Barber, Cheryl ; Abrams, William ; Da Costa, Antonio Charlys ; De Pádua Milagres, Flavio Augusto ; Braz-Silva, Paulo Henrique ; Malamud, Daniel ; Gallottini, Marina</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c590t-4ca28dc3eb24354b9e7a10a7c43e5f2907c1481590bfa83779b6426d67410c7a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Blood</topic><topic>Dengue</topic><topic>Dentistry</topic><topic>Flavivirus</topic><topic>Guillain-Barre syndrome</topic><topic>infection</topic><topic>Infections</topic><topic>LAMP</topic><topic>Nucleic acids</topic><topic>Original</topic><topic>Polymerase chain reaction</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA viruses</topic><topic>RNA-directed DNA polymerase</topic><topic>Saliva</topic><topic>Studies</topic><topic>Thermal cycling</topic><topic>Urine</topic><topic>Vector-borne diseases</topic><topic>virology</topic><topic>Viruses</topic><topic>Zika</topic><topic>Zika virus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Castro, Talita</creatorcontrib><creatorcontrib>Sabalza, Maite</creatorcontrib><creatorcontrib>Barber, Cheryl</creatorcontrib><creatorcontrib>Abrams, William</creatorcontrib><creatorcontrib>Da Costa, Antonio Charlys</creatorcontrib><creatorcontrib>De Pádua Milagres, Flavio Augusto</creatorcontrib><creatorcontrib>Braz-Silva, Paulo Henrique</creatorcontrib><creatorcontrib>Malamud, Daniel</creatorcontrib><creatorcontrib>Gallottini, Marina</creatorcontrib><collection>Taylor &amp; Francis Open Access(OpenAccess)</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium &amp; Calcified Tissue Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>ProQuest - Health &amp; Medical Complete保健、医学与药学数据库</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>ProQuest Biological Science Journals</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals</collection><jtitle>Journal of oral microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Castro, Talita</au><au>Sabalza, Maite</au><au>Barber, Cheryl</au><au>Abrams, William</au><au>Da Costa, Antonio Charlys</au><au>De Pádua Milagres, Flavio Augusto</au><au>Braz-Silva, Paulo Henrique</au><au>Malamud, Daniel</au><au>Gallottini, Marina</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid diagnosis of Zika virus through saliva and urine by Loop-mediated isothermal amplification (LAMP)</atitle><jtitle>Journal of oral microbiology</jtitle><addtitle>J Oral Microbiol</addtitle><date>2018-01-01</date><risdate>2018</risdate><volume>10</volume><issue>1</issue><spage>1510712</spage><epage>6</epage><pages>1510712-6</pages><issn>2000-2297</issn><eissn>2000-2297</eissn><abstract>Background: Zika virus (ZIKV) is a single-stranded RNA virus and member of the Flaviviridae family. Recent studies have reported that saliva can be an important alternative to detect ZIKV. Saliva requires less processing than blood greatly simplifying the assay. Loop-mediated Isothermal Amplification (LAMP) is a rapid assay that detects nucleic acids, including ZIKV RNA. Aim: The aim of this study was to evaluate the efficacy of saliva and urine to diagnose ZIKV infection in subjects during the acute phase, through ZIKV RNA detection by LAMP. Method: A total of 131 samples (68 saliva and 63 urine samples) from 69 subjects in the acute phase of ZIKV infection, and confirmed positive for ZIKV by blood analysis through real time-PCR, were collected and analyzed by Reverse Transcriptase Loop-mediated Isothermal Amplification (RT-LAMP). Results: From the 68 saliva samples, 45 (66.2%) were positive for ZIKV with an average time to positivity (Tp) of 13.5 min, and from the 63 urine samples, 25 (39.7%) were positive with the average Tp of 15.8 min. Saliva detected more samples (p = 0.0042) and had faster Tp (p = 0.0176) as compared with urine. Conclusion: Saliva proved to be a feasible alternative to diagnose ZIKV infection during the acute phase by LAMP.</abstract><cop>United States</cop><pub>Taylor &amp; Francis</pub><pmid>30202506</pmid><doi>10.1080/20002297.2018.1510712</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0001-5516-5177</orcidid><orcidid>https://orcid.org/0000-0003-4019-376X</orcidid><orcidid>https://orcid.org/0000-0002-1842-9521</orcidid><orcidid>https://orcid.org/0000-0002-2159-507X</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 2000-2297
ispartof Journal of oral microbiology, 2018-01, Vol.10 (1), p.1510712-6
issn 2000-2297
2000-2297
language eng
recordid cdi_proquest_miscellaneous_2102327942
source Publicly Available Content Database; PubMed Central(OpenAccess); Taylor & Francis Open Access(OpenAccess)
subjects Blood
Dengue
Dentistry
Flavivirus
Guillain-Barre syndrome
infection
Infections
LAMP
Nucleic acids
Original
Polymerase chain reaction
Ribonucleic acid
RNA
RNA viruses
RNA-directed DNA polymerase
Saliva
Studies
Thermal cycling
Urine
Vector-borne diseases
virology
Viruses
Zika
Zika virus
title Rapid diagnosis of Zika virus through saliva and urine by Loop-mediated isothermal amplification (LAMP)
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-01T09%3A41%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_infor&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Rapid%20diagnosis%20of%20Zika%20virus%20through%20saliva%20and%20urine%20by%20Loop-mediated%20isothermal%20amplification%20(LAMP)&rft.jtitle=Journal%20of%20oral%20microbiology&rft.au=Castro,%20Talita&rft.date=2018-01-01&rft.volume=10&rft.issue=1&rft.spage=1510712&rft.epage=6&rft.pages=1510712-6&rft.issn=2000-2297&rft.eissn=2000-2297&rft_id=info:doi/10.1080/20002297.2018.1510712&rft_dat=%3Cproquest_infor%3E2102327942%3C/proquest_infor%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c590t-4ca28dc3eb24354b9e7a10a7c43e5f2907c1481590bfa83779b6426d67410c7a3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2171121349&rft_id=info:pmid/30202506&rfr_iscdi=true