Adjuvant effect of water-soluble polysaccharide (PAP) from the mycelium of Polyporus albicans on the immune responses to ovalbumin in mice

Abstract In our previous work, a new water-soluble polysaccharide (PAP) from the mycelium of Polyporus albicans was identified for the first time. Preliminary tests in vitro showed PAP have potent stimulating effects on murine lymphocyte proliferation induced by mitogen. So in this study, the immuno...

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Bibliographic Details
Published in:Vaccine 2008-07, Vol.26 (31), p.3932-3936
Main Authors: Sun, Yongxu, Liu, Jicheng
Format: Article
Language:English
Subjects:
Adjuvants
Adjuvants, Immunologic - administration & dosage
Adjuvants, Immunologic - isolation & purification
Adjuvants, Immunologic - pharmacology
Allergy and Immunology
Alum Compounds - administration & dosage
Alum Compounds - pharmacology
Animals
Antibodies - blood
Antibody
Applied microbiology
Biological and medical sciences
Cell Proliferation
Fundamental and applied biological sciences. Psychology
Immunoglobulin G - blood
Lymphocytes - immunology
Male
Mice
Mice, Inbred ICR
Microbiology
Miscellaneous
Mycelium - chemistry
Mycology
Ovalbumin - immunology
Polyporaceae - chemistry
Polyporus
Polyporus albicans
Polysaccharide
Polysaccharides - administration & dosage
Polysaccharides - isolation & purification
Polysaccharides - pharmacology
Polysaccharides - toxicity
Proliferation
Spleen - immunology
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
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Summary:Abstract In our previous work, a new water-soluble polysaccharide (PAP) from the mycelium of Polyporus albicans was identified for the first time. Preliminary tests in vitro showed PAP have potent stimulating effects on murine lymphocyte proliferation induced by mitogen. So in this study, the immunomodulatory effect and the adjuvant potential of PAP on the cellular and humoral immune response of ICR mice against ovalbumin were investigated. In vivo toxicity assays of PAP showed not to be lethal for mice in doses ranging from 0.5 to 4 mg. ICR mice were immunized subcutaneously with OVA 0.1 mg alone or with OVA 0.1 mg dissolved in saline containing alum (0.2 mg) or PAP (0.5, 1 and 2 mg) on days 1 and 15. Two weeks later (day 28), concanavalin A (Con A)-, lipopolysaccharide (LPS)- and OVA-stimulated splenocyte proliferation and OVA-specific serum antibodies were measured. PAP (0.5, 1 and 2 mg) other than alum significantly enhanced the Con A-, LPS- or OVA-induced splenocyte proliferation in the OVA-immunized mice ( P < 0.05 or
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2008.04.018