Genetic variability in Aedes aegypti (L.) (Diptera: Culicidae) populations using RAPD markers

Aedes aegypti (L.) is an important vector of diseases such as the yellow fever and dengue, present in tropical and subtropical regions. The objective of this study was to analyze the genetic variability of different A. aegypti populations using RAPD (Random Amplified Polymorphic DNA) markers as a ba...

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Bibliographic Details
Published in:Neotropical entomology 2009-07, Vol.38 (4), p.542
Main Authors: Hiragi, Cassia, Simões, Kenya, Martins, Erica, Queiroz, Paulo, Lima, Luzia, Monnerat, Rose
Format: Article
Language:Portuguese
Subjects:
Aedes aegypti
Animals
Culicidae
Culicidae - genetics
Diptera
Genetic Variation
Random Amplified Polymorphic DNA Technique
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Summary:Aedes aegypti (L.) is an important vector of diseases such as the yellow fever and dengue, present in tropical and subtropical regions. The objective of this study was to analyze the genetic variability of different A. aegypti populations using RAPD (Random Amplified Polymorphic DNA) markers as a basic study to support the use of biocontrol strategies. DNA of ten collected larvae from three different populations were analyzed using ten RAPD primers. The results indicated the existence of genetic variability inter and intrapopulation. This was confirmed by a dendrogram that grouped the populations in two main clusters with a genetic similarity of 24%. In one of these clusters, it was possible to distinguish two populations that showed 50% similarity. The molecular variance analysis indicated that the interpopulation genetic diversity (55,01%) was higher than the intrapopulation genetic diversity (44,99%). A high genetic polymorphism (Ht = 0.2656) and high levels of genetic differentiation between populations (Gst = 0.3689) were found. The adopted DNA extraction protocol proved to be efficient regardless the insect development stage used, avoiding the addition of reagents or additional stages of processing. Future experiments can be performed to confirm if the detected variability is related to the resistance characteristics of each population to a determined pesticide.
ISSN:1519-566X
DOI:10.1590/S1519-566X2009000400018