Phosphoinositide‐dependent kinase 1–associated glycolysis is regulated by miR‐409‐3p in clear cell renal cell carcinoma

• Published in: Journal of cellular biochemistry 2019-01, Vol.120 (1), p.126-134
• Main Authors: Wang, Yongjun, He, Yanfa, Bai, Hongzhong, Dang, Yi, Gao, Jiangyan, Lv, Pei
• Format: Article
• Language: English
• Subjects: 3-Phosphoinositide-Dependent Protein Kinases - genetics; 3-Phosphoinositide-Dependent Protein Kinases - metabolism; Adults; Carcinoma, Renal Cell - metabolism; Carcinoma, Renal Cell - pathology; Carcinoma, Renal Cell - surgery; Cell Hypoxia; Cell Line, Tumor; Cell Proliferation; clear cell renal cell carcinoma; Clear cell-type renal cell carcinoma; Gene Expression Regulation, Neoplastic; Gene Knockdown Techniques; Glucose - metabolism; Glycolysis; Humans; Hypoxia; Hypoxia-Inducible Factor 1, alpha Subunit - metabolism; Kidney cancer; Kidney Neoplasms - metabolism; Kidney Neoplasms - pathology; Kidney Neoplasms - surgery; Kidneys; Metabolic activation; Metabolic rate; Metabolism; MicroRNAs - chemistry; MicroRNAs - metabolism; miRNA; miR‐409‐3p; Molecular Mimicry - genetics; Oxygen Consumption; phosphoinositide‐dependent kinase 1; Regulators; Transfection
• ISSN: 0730-2312; 1097-4644
• DOI: 10.1002/jcb.27152

Clear cell renal cell carcinoma (ccRCC) is the most popular kidney cancer in adults. Metabolic shift toward aerobic glycolysis is a fundamental factor for ccRCC therapy. MicroRNAs (miRNAs) are thought to be important regulators in ccRCC development and progression. Phosphoinositide‐dependent kinase 1 (PDK1) is required for metabolic activation; however, the role of PDK1‐induced glycolytic metabolism regulated by miRNAs is unclear in ccRCC. So, the purpose of the current study is to elucidate the underlying mechanism in ccRCC cell metabolism mediated by PDK1. Our results revealed that miR‐409‐3p inhibited glycolysis by regulating PDK1 expression in ccRCC cells. We also found that miR‐409‐3p was regulated by hypoxia. Our results indicated that PDK1 facilitated ccRCC cell glycolysis, regulated by miR‐409‐3p in hypoxia. MiR‐409‐3p is a target gene of phosphoinositide‐dependent kinase 1 in clear cell renal cell carcinoma (ccRCC) cells and tissues, which is downregulated by hypoxia/hypoxia‐inducible factor 1‐α. MiR‐409‐3p decreases ccRCC cell survival ability and glycolysis.